http://hdl.handle.net/2440/10015 2013-05-23T06:43:16Z 2013-05-23T06:43:16Z Tran, Hai Bac Lewis, Martin David Tan, Lor-Wai Lester, Susan Elizabeth Baker, Leonie Michelle Ng, Jia Qi Hamilton-Bruce, Monica Anne Hill, Catherine Louise Koblar, Simon Andrea Rischmueller, Maureen Ruffin, Richard Ernest Wormald, Peter-John Zalewski, Peter David Lang, Carol Jane http://hdl.handle.net/2440/77724 2013-05-17T01:47:09Z 2011-12-31T13:30:00Z

Title: Immunolocalization of NLRP3 inflammasome in normal murine airway epithelium and changes following induction of ovalbumin-induced airway inflammation Author: Tran, Hai Bac; Lewis, Martin David; Tan, Lor-Wai; Lester, Susan Elizabeth; Baker, Leonie Michelle; Ng, Jia Qi; Hamilton-Bruce, Monica Anne; Hill, Catherine Louise; Koblar, Simon Andrea; Rischmueller, Maureen; Ruffin, Richard Ernest; Wormald, Peter-John; Zalewski, Peter David; Lang, Carol Jane Abstract: Little is known about innate immunity and components of inflammasomes in airway epithelium. This study evaluated immunohistological evidence for NLRP3 inflammasomes in normal and inflamed murine (Balb/c) airway epithelium in a model of ovalbumin (OVA) induced allergic airway inflammation. The airway epithelium of control mice exhibited strong cytoplasmic staining for total caspase-1, ASC, and NLRP3, whereas the OVA mice exhibited strong staining for active caspase-1, with redistribution of caspase-1, IL-1β and IL-18, indicating possible activation of the NLRP3 inflammasome. Active caspase-1, NLRP3, and other inflammasome components were also detected in tissue eosinophils from OVA mice, and may potentially contribute to IL-1β and IL-18 production. In whole lung, inRNA expression of NAIP and procaspase-1 was increased in OVA mice, whereas NLRP3, IL-1β and IL-18 decreased. Some OVA-treated mice also had significantly elevated and tightly correlated serum levels of IL-1β and TNFα. In cultured normal human bronchial epithelial cells, LPS priming resulted in a significant increase in NLRP3 and II-lp protein expression. This study is the first to demonstrate NLRP3 inflammasome components in normal airway epithelium and changes with inflammation. We propose activation and/or luminal release of the inflammasome is a feature of allergic airway inflammation which may contribute to disease pathogenesis. Description: Extent: 18p.

2011-12-31T13:30:00Z Ryall, Rosemary Lyons Grover, Phulwinder Kumar Thurgood, Lauren A. Chauvet, Magali C. Fleming, David E. van Bronswijk, Wilhelm http://hdl.handle.net/2440/77188 2013-04-24T04:30:25Z 2006-12-31T13:30:00Z

Title: The importance of a clean face: the effect of different washing procedures on the association of Tamm-Horsfall glycoprotein and other urinary proteins with calcium oxalate crystals Author: Ryall, Rosemary Lyons; Grover, Phulwinder Kumar; Thurgood, Lauren A.; Chauvet, Magali C.; Fleming, David E.; van Bronswijk, Wilhelm

2006-12-31T13:30:00Z Psaltis, Alkiviadis James Foreman, Andrew Wormald, Peter-John Schlosser, Rodney J. http://hdl.handle.net/2440/76222 2013-03-25T05:34:22Z 2011-12-31T13:30:00Z

Title: Contamination of sinus irrigation devices: A review of the evidence and clinical relevance Author: Psaltis, Alkiviadis James; Foreman, Andrew; Wormald, Peter-John; Schlosser, Rodney J. Abstract: Background: The use of saline and topical medications in the treatment of chronic rhinosinusitis (CRS) is now common practice among otolaryngologists. Although high-level studies have consistently shown the benefit of saline in symptom improvement of CRS patients, to date, only lower-level evidence exists advocating a benefit of other topical medications. Methods: Recently, increased interest has emerged surrounding the devices used to deliver such therapies with mounting evidence consistently showing contamination with pathogenic organisms such as Staphylococcus aureus and Pseudomonas aeruginosa. Results: In addition, the potential for such contamination to cross-infect sinus cavities and lead to postoperative infections is also of concern. Conclusion: This review examines the current evidence available concerning the issue of contamination and its clinical relevance and offers the otolaryngologist advice on patient education when dispensing such devices.

2011-12-31T13:30:00Z Tiong, Leong Ung Finnie, John Walker Field, John Benjamin Francis Maddern, Guy John http://hdl.handle.net/2440/76221 2013-03-25T05:34:16Z 2011-12-31T13:30:00Z

Title: Bimodal Electric Tissue Ablation (BETA): A study on ablation size when the anode is placed on the peritoneum and the liver Author: Tiong, Leong Ung; Finnie, John Walker; Field, John Benjamin Francis; Maddern, Guy John

2011-12-31T13:30:00Z