http://hdl.handle.net/2440/11376 2013-06-18T21:17:39Z 2013-06-18T21:17:39Z Rousseau, Mathieu Andre Jean-Marie Ayliffe, Michael Anthony Timmis, Jeremy Newman http://hdl.handle.net/2440/74533 2012-12-03T04:30:48Z 2011-12-31T13:30:00Z

Title: Plastid DNA in the nucleus: new genes for old Author: Rousseau, Mathieu Andre Jean-Marie; Ayliffe, Michael Anthony; Timmis, Jeremy Newman Abstract: Nuclear genomes of eukaryotes are bombarded by a continuous deluge of organellar DNA which contributes significantly to eukaryote evolution. Here, we present a new PCR-based method that allows the specific amplification of nuclear integrants of organellar DNA (norgs) by exploiting recent deletions present in organellar genome sequences. We have used this method to amplify nuclear integrants of plastid DNA (nupts) from the nuclear genomes of several nicotiana species and to study the evolutionary forces acting upon these sequences. The role of nupts in endosymbiotic evolution and the different genetic factors influencing the time available for a chloroplastic gene to be functionally relocated in the nucleus are discussed.

2011-12-31T13:30:00Z Neilsen, Corine T. Goodall, Gregory John Bracken, Cameron Peter http://hdl.handle.net/2440/74498 2012-12-02T23:30:31Z 2011-12-31T13:30:00Z

Title: IsomiRs - the overlooked repertoire in the dynamic microRNAome Author: Neilsen, Corine T.; Goodall, Gregory John; Bracken, Cameron Peter Abstract: The development of deep sequencing has enabled the identification of novel microRNAs (miRNAs), leading to a growing appreciation for the fact that individual miRNAs can be heterogeneous in length and/or sequence. These variants, termed isomiRs, can be expressed in a cell-specific manner, and numerous recent studies suggest that at least some isomiRs may affect target selection, miRNA stability, or loading into the RNA-induced silencing complex (RISC). Reports indicating differential functionality for isomiRs are currently confined to several specific variants, and although isomiRs are common, their broader biological significance is yet to be fully resolved. Here we review the growing body of evidence suggesting that isomiRs have functional differences, of which at least some appear biologically relevant, and caution researchers to take miRNA isoforms into consideration in their experiments.

2011-12-31T13:30:00Z Newman, Morgan Nornes, Svanhild Martins, Ralph Nigel Lardelli, Michael Trent http://hdl.handle.net/2440/72801 2012-08-27T06:30:19Z 2011-12-31T13:30:00Z

Title: Robust homeostasis of Presenilin1 protein levels by transcript regulation Author: Newman, Morgan; Nornes, Svanhild; Martins, Ralph Nigel; Lardelli, Michael Trent

2011-12-31T13:30:00Z Wang, Dong Lloyd, Andrew Henry Mark Timmis, Jeremy Newman http://hdl.handle.net/2440/72444 2012-08-09T04:30:37Z 2011-12-31T13:30:00Z

Title: Nuclear genome diversity in somatic cells is accelerated by environmental stress Author: Wang, Dong; Lloyd, Andrew Henry Mark; Timmis, Jeremy Newman

2011-12-31T13:30:00Z