http://hdl.handle.net/2440/10016 Sat, 25 May 2013 21:12:07 GMT 2013-05-25T21:12:07Z http://hdl.handle.net/2440/77724 Title: Immunolocalization of NLRP3 inflammasome in normal murine airway epithelium and changes following induction of ovalbumin-induced airway inflammation Author: Tran, Hai Bac; Lewis, Martin David; Tan, Lor-Wai; Lester, Susan Elizabeth; Baker, Leonie Michelle; Ng, Jia Qi; Hamilton-Bruce, Monica Anne; Hill, Catherine Louise; Koblar, Simon Andrea; Rischmueller, Maureen; Ruffin, Richard Ernest; Wormald, Peter-John; Zalewski, Peter David; Lang, Carol Jane Abstract: Little is known about innate immunity and components of inflammasomes in airway epithelium. This study evaluated immunohistological evidence for NLRP3 inflammasomes in normal and inflamed murine (Balb/c) airway epithelium in a model of ovalbumin (OVA) induced allergic airway inflammation. The airway epithelium of control mice exhibited strong cytoplasmic staining for total caspase-1, ASC, and NLRP3, whereas the OVA mice exhibited strong staining for active caspase-1, with redistribution of caspase-1, IL-1β and IL-18, indicating possible activation of the NLRP3 inflammasome. Active caspase-1, NLRP3, and other inflammasome components were also detected in tissue eosinophils from OVA mice, and may potentially contribute to IL-1β and IL-18 production. In whole lung, inRNA expression of NAIP and procaspase-1 was increased in OVA mice, whereas NLRP3, IL-1β and IL-18 decreased. Some OVA-treated mice also had significantly elevated and tightly correlated serum levels of IL-1β and TNFα. In cultured normal human bronchial epithelial cells, LPS priming resulted in a significant increase in NLRP3 and II-lp protein expression. This study is the first to demonstrate NLRP3 inflammasome components in normal airway epithelium and changes with inflammation. We propose activation and/or luminal release of the inflammasome is a feature of allergic airway inflammation which may contribute to disease pathogenesis. Description: Extent: 18p. Sat, 31 Dec 2011 13:30:00 GMT http://hdl.handle.net/2440/77724 2011-12-31T13:30:00Z http://hdl.handle.net/2440/77188 Title: The importance of a clean face: the effect of different washing procedures on the association of Tamm-Horsfall glycoprotein and other urinary proteins with calcium oxalate crystals Author: Ryall, Rosemary Lyons; Grover, Phulwinder Kumar; Thurgood, Lauren A.; Chauvet, Magali C.; Fleming, David E.; van Bronswijk, Wilhelm Sun, 31 Dec 2006 13:30:00 GMT http://hdl.handle.net/2440/77188 2006-12-31T13:30:00Z http://hdl.handle.net/2440/76222 Title: Contamination of sinus irrigation devices: A review of the evidence and clinical relevance Author: Psaltis, Alkiviadis James; Foreman, Andrew; Wormald, Peter-John; Schlosser, Rodney J. Abstract: Background: The use of saline and topical medications in the treatment of chronic rhinosinusitis (CRS) is now common practice among otolaryngologists. Although high-level studies have consistently shown the benefit of saline in symptom improvement of CRS patients, to date, only lower-level evidence exists advocating a benefit of other topical medications. Methods: Recently, increased interest has emerged surrounding the devices used to deliver such therapies with mounting evidence consistently showing contamination with pathogenic organisms such as Staphylococcus aureus and Pseudomonas aeruginosa. Results: In addition, the potential for such contamination to cross-infect sinus cavities and lead to postoperative infections is also of concern. Conclusion: This review examines the current evidence available concerning the issue of contamination and its clinical relevance and offers the otolaryngologist advice on patient education when dispensing such devices. Sat, 31 Dec 2011 13:30:00 GMT http://hdl.handle.net/2440/76222 2011-12-31T13:30:00Z http://hdl.handle.net/2440/76221 Title: Bimodal Electric Tissue Ablation (BETA): A study on ablation size when the anode is placed on the peritoneum and the liver Author: Tiong, Leong Ung; Finnie, John Walker; Field, John Benjamin Francis; Maddern, Guy John Sat, 31 Dec 2011 13:30:00 GMT http://hdl.handle.net/2440/76221 2011-12-31T13:30:00Z