Tobacco mosaic virus RNA as an internal control for duplex RT-PCR assay of pea germplasm
Date
2001
Authors
Torok, V. A.
Randles, John Wesley
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Type:
Journal article
Citation
Australasian Plant Pathology, 2001; 30 (3):227-230
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Abstract
Tobacco mosaic virus (TMV) is an easily prepared, plentiful and stable source of homogenous RNA suitable for addition to reverse transcription polymerase chain reaction (RT-PCR) assays as an internal control. We describe primers and conditions used in a duplex assay for Pea seedborne mosaic virus (PSbMV)-infection of pea seed as an example of the utility of the system for RT-PCR screening of plant germplasm for the presence or absence of a virus.
School/Discipline
School of Agriculture, Food and Wine : Plant and Food Science
School of Agriculture, Food and Wine : Agricultural and Animal Science
School of Agriculture, Food and Wine : Agricultural and Animal Science