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Adelaide Research and Scholarship
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Schools and Disciplines
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School of Agriculture, Food and Wine
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Agriculture, Food and Wine Publications
Permanent link to this item:
http://hdl.handle.net/2440/27788
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| Type: | Article |
| Title: | Insect hemolymph clotting : evidence for interaction between the coagulation system and the prophenoloxidase activating cascade |
| Author: | Li, D.
Scherfer, C.
Korayem, A. M.
Zhao, Zeng Qi
Schmidt, Otto
Theopold, U. J. |
| Citation: | Insect Biochemistry and Molecular Biology, 2002; 32 (8):919-928 |
| Publisher: | Pergamon-Elsevier |
| Issue Date: | 2002 |
| ISSN: | 0965-1748 |
| School/Discipline: | Applied and Molecular Ecology
School of Agriculture, Food and Wine : Agricultural and Animal Science |
| Abstract: | Here we describe a novel approach to isolate proteins involved in insect hemolymph coagulation. In order to avoid problems in purifying clot proteins after they had been crosslinked, we performed an in vitro coagulation reaction with cell-free hemolymph from the lepidopteran Galleria mellonella and used the resulting complexes to produce a specific antiserum. The antiserum reacted with a subset of hemolymph proteins as well as with granular cells, but not with other hemocyte types of Galleria. Screening expression libraries identified some positive clones, which turned out to code for some previously characterized components of immune cascades, as well as some novel candidates for clotting factors. Known components include members of both the coagulation system and the prophenol-activating cascade, lending support to the idea that both systems work together during the formation of a hemolymph clot. Novel candidates for insect clotting factors include a mucin-like protein, a glutathione-S-transferase, and a distant member of the α-crystallin/small heat shock protein family. Using assays measuring the activity of transglutaminase, a key enzyme in clotting reactions in both vertebrates and invertebrates, we found a partial overlap between transglutaminase substrates and proteins recognized by the antiserum against the in vitro-induced clot. |
| Keywords: | Insect immunity; hemolymph coagulation; clotting; transglutaminase; lipophorin; prophenoloxidase; glutathione-S-transferase; α-crystallin; galleria mellonella |
| RMID: | 0020020522 |
| DOI: | 10.1016/S0965-1748(02)00030-9 |
| Description (link): | http://www.elsevier.com/wps/find/journaldescription.cws_home/390/description#description |
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| Appears in Collections: | Agriculture, Food and Wine Publications
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