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https://hdl.handle.net/2440/56778
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Type: | Journal article |
Title: | Dietary omega-3 polyunsaturated fatty acid does not influence the intestinal microbial communities of broiler chickens |
Author: | Geier, M. Torok, V. Allison, G. Ophel-Keller, K. Gibson, R. Munday, C. Hughes, R. |
Citation: | Poultry Science, 2009; 88(11):2399-2405 |
Publisher: | Poultry Science Assoc Inc |
Issue Date: | 2009 |
ISSN: | 0032-5791 1525-3171 |
Statement of Responsibility: | M. S. Geier, V. A. Torok, G. E. Allison, K. Ophel-Keller, R. A. Gibson, C. Munday and R. J. Hughes |
Abstract: | The capacity for n-3 polyunsaturated fatty acids (PUFA) to improve broiler chicken growth, influence the intestinal microbial communities, and modify the PUFA content of meat was studied. Male Cobb 500 chickens were fed 1 of 4 diets from hatch: control (standard diet with no additives), ZnB (standard diet with added antibiotics), 2% SALmate (standard diet with 2% SALmate, which is composed of 42% fish oil and 58% starch), and 5% SALmate (standard diet with 5% SALmate). A 7-d energy metabolism study was conducted between d 15 and 22 posthatch. Birds were killed at d 25 and intestinal samples were collected to assess microbial communities by terminal restriction fragment length polymorphism and Lactobacillus PCR-denaturing gradient gel electrophoresis. Diet did not affect BW, feed intake, feed conversion, or ileal digestible energy (P > 0.05). Apparent ME was greater in ZnB-fed birds compared with all other diets (P < 0.05). Breast tissue levels of eicosapentaenoic acid, docosahexaenoic acid, docosapentaenoic acid, and total n-3 PUFA were elevated significantly in 2% SALmate- and 5% SALmate-fed chickens compared with control and ZnB diets (P < 0.05). No significant differences in overall microbial communities were observed in the ileum or cecum as assessed by terminal RFLP (P > 0.05). Birds fed 2% SALmate had a significantly different cecal Lactobacillus species profile compared with birds fed the control diet (P < 0.05); however, no differences were observed in birds fed 5% SALmate compared with birds fed all other diets. In addition to the expected increase in breast tissue n-3 fatty acid levels, a low level of dietary n-3 PUFA also altered the intestinal Lactobacillus species profiles. However, n-3 PUFA supplementation did not alter the overall microbial communities or broiler performance. |
Keywords: | broiler denaturing gradient gel electrophoresis polyunsaturated fatty acid terminal restriction fragment length polymorphism |
Description: | © 2009 Poultry Science Association |
DOI: | 10.3382/ps.2009-00126 |
Published version: | http://dx.doi.org/10.3382/ps.2009-00126 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 5 |
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