Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/69808
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Type: | Journal article |
Title: | Adipokines and adipocyte function in clock mutant mice that retain melatonin rhythmicity |
Author: | Kennaway, D. Owens, J. Voultsios, A. Wight, N. |
Citation: | Obesity, 2012; 20(2):295-305 |
Publisher: | North Amer Assoc Study Obesity |
Issue Date: | 2012 |
ISSN: | 1930-7381 1930-739X |
Statement of Responsibility: | David J. Kennaway, Julie A. Owens, Athena Voultsios and Nicole Wight |
Abstract: | Clockδ19+MEL mutant mice, which retain melatonin rhythmicity, but lack peripheral tissue rhythmicity have impaired glucose tolerance, but reduced plasma free fatty acids, increased plasma adiponectin, and improved insulin sensitivity. Here, we report their response to a high-fat diet and adipocyte rhythmicity and function. The diet increased epigonadal fat weight similarly (twofold) in both wild-type and Clockδ19+MEL mice. The Clockδ19 mutation abolished rhythmicity of Per2, Rev erbα and peroxisome proliferator-activated receptor-γ (Pparγ ) mRNA in epigonadal fat, but not Bmal1 mRNA, and reduced Rev erbα mRNA by 59 and 70% compared to the wild-type mice on the control and high-fat diets, respectively. The mutants had increased Adipoq mRNA expression in epigonadal fat (22%; P < 0.05) on a control diet, but showed no further change on a high-fat diet, and no change in Lep, Nampt or Retn mRNA on either diet. The Clockδ19 mutation abolished rhythmicity of genes in epigonadal fat that contribute to plasma free fatty acids for mice on both diets, and increased Lipe mRNA expression in those on the high-fat diet. The persistent melatonin rhythm and reduced plasma free fatty acids in Clockδ19+MEL mutants may contribute to their enhanced insulin sensitivity, ameliorate the extent of impaired glucose homeostasis, and protect against the adverse effects of a high-fat diet. |
Keywords: | Animals Mice Insulin Resistance Fatty Acids, Nonesterified PPAR alpha Gene Expression Regulation Energy Metabolism Periodicity Mutation Female Male Adiposity CLOCK Proteins Diet, High-Fat |
Rights: | © 2012 North American Association for the Study of Obesity (NAASO) |
DOI: | 10.1038/oby.2011.276 |
Published version: | http://dx.doi.org/10.1038/oby.2011.276 |
Appears in Collections: | Aurora harvest Obstetrics and Gynaecology publications |
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hdl_69808.pdf | Accepted version | 645.44 kB | Adobe PDF | View/Open |
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