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Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/71093

Type: Book chapter
Title: Chromosome Analysis in Invertebrates and Vertebrates
Author: Rowell, David M.
Lim, Shu Ly
Grutzner, Frank
Citation: Molecular Methods for Evolutionary Genetics, 2011 / V. Orgogozo & M. V. Rockman (eds.), Ch. 2 pp.13-35
Publisher: Springer
Issue Date: 2011
Series/Report no.: Methods in Molecular Biology ; 772
ISBN: 9781617792274
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School/Discipline: School of Molecular and Biomedical Science : Genetics
Statement of
Responsibility: 
David M. Rowell, Shu Ly Lim, and Frank Grutzner
Abstract: The revolution in molecular techniques over the last 30 years detracted from many traditional cytological techniques for examining basic biological problems. One of these casualties is the preparation of karyotypes and analysis of chromosomal structure, behaviour, and variation. Recent technology permitting the full sequencing of organisms has highlighted (but does not replace) the importance of understanding chromosomal constitution and karyotype structure, which underpin genome organisation. This chapter provides simple and straightforward protocols for the preparation of chromosome spreads from animals, and more advanced techniques for cell culture and chromosomal banding and hybridisation.
Keywords: Chromosomes; Invertebrate genetics; Cytological techniques; Karyology; Cell culture; Fluorescence in situ hybridisation
Rights: © Springer Science+Business Media, LLC 2011
RMID: 0020114812
DOI: 10.1007/978-1-61779-228-1_2
Appears in Collections:Environment Institute Leaders publications
Genetics publications
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