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Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/73306

Type: Journal article
Title: Bovine ncRNAs are abundant, primarily intergenic, conserved and associated with regulatory genes
Author: Qu, Zhipeng
Adelson, David Louis
Citation: PLoS ONE, 2012; 7(8):e42638
Publisher: Public Library of Science
Issue Date: 2012
ISSN: 1932-6203
School/Discipline: School of Molecular and Biomedical Science
Statement of
Responsibility: 
Zhipeng Qu and David L. Adelson
Abstract: It is apparent that non-coding transcripts are a common feature of higher organisms and encode uncharacterized layers of genetic regulation and information. We used public bovine EST data from many developmental stages and tissues, and developed a pipeline for the genome wide identification and annotation of non-coding RNAs (ncRNAs). We have predicted 23,060 bovine ncRNAs, 99% of which are un-annotated, based on known ncRNA databases. Intergenic transcripts accounted for the majority (57%) of the predicted ncRNAs and the occurrence of ncRNAs and genes were only moderately correlated (r = 0.55, p-value<2.2e-16). Many of these intergenic non-coding RNAs mapped close to the 3’ or 5’ end of thousands of genes and many of these were transcribed from the opposite strand with respect to the closest gene, particularly regulatory-related genes. Conservation analyses showed that these ncRNAs were evolutionarily conserved, and many intergenic ncRNAs proximate to genes contained sequence-specific motifs. Correlation analysis of expression between these intergenic ncRNAs and protein-coding genes using RNA-seq data from a variety of tissues showed significant correlations with many transcripts. These results support the hypothesis that ncRNAs are common, transcribed in a regulated fashion and have regulatory functions.
Rights: Copyright: © 2012 Qu, Adelson. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
RMID: 0020121499
DOI: 10.1371/journal.pone.0042638
Appears in Collections:Environment Institute Publications
Molecular and Biomedical Science Publications
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