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Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/73371

Type: Journal article
Title: Self-association of the Shigella flexneri IcsA autotransporter protein
Author: May, K.
Grabowicz, M.
Polyak, S.
Morona, R.
Citation: Microbiology, 2012; 158(7):1874-1883
Publisher: Soc General Microbiology
Issue Date: 2012
ISSN: 1350-0872
1465-2080
Statement of
Responsibility: 
Kerrie L. May, Marcin Grabowicz, Steven W. Polyak and Renato Morona
Abstract: The IcsA autotransporter protein is a major virulence factor of the human intracellular pathogen Shigella flexneri. IcsA is distributed at the poles in the outer membrane (OM) of S. flexneri and interacts with components of the host actin-polymerization machinery to facilitate intracellular actin-based motility and subsequent cell-to-cell spreading of the bacterium. We sought to characterize the biochemical properties of IcsA in the bacterial OM. Chemical cross-linking data suggested that IcsA exists in a complex in the OM. Furthermore, reciprocal co-immunoprecipitation of differentially epitope-tagged IcsA proteins indicated that IcsA is able to self-associate. The identification of IcsA linker-insertion mutants that were negatively dominant provided genetic evidence of IcsA–IcsA interactions. From these results, we propose a model whereby IcsA self-association facilitates efficient actin-based motility.
Keywords: Shigella flexneri; Bacterial Proteins; Bacterial Outer Membrane Proteins; DNA-Binding Proteins; Transcription Factors; Virulence Factors; Cross-Linking Reagents; Immunoprecipitation; Protein Binding; Protein Multimerization
Rights: © 2012 SGM
RMID: 0020120711
DOI: 10.1099/mic.0.056465-0
Appears in Collections:Molecular and Biomedical Science publications
IPAS publications
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