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Microbiology and Immunology Publications
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|Type: ||Journal article|
|Title: ||Escherichia coli 83972 expressing a P fimbriae oligosaccharide receptor mimic impairs adhesion of uropathogenic E. coli|
|Author: ||Watts, Rebecca E.|
Tan, Chee K.
Ulett, Glen C.
Carey, Alison J.
Paton, Adrienne Webster
Paton, James Cleland
Schembri, Mark A.
|Citation: ||Journal of Infectious Diseases, 2012; 206 (8):1242-1249|
|Publisher: ||Oxford University Press on behalf of the Infectious Diseases Society of America|
|Issue Date: ||2012|
|School/Discipline: ||School of Molecular and Biomedical Science : Microbiology and Immunology|
|Rebecca E. Watts, Chee K. Tan, Glen C. Ulett, Alison J. Carey, Makrina Totsika, Adi Idris, Adrienne W. Paton, Renato Morona, James C. Paton, and Mark A. Schembri|
|Abstract: ||Urinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) are a significant health concern, exacerbated by the rapid emergence of multidrug resistant strains refractory to antibiotic treatment. P fimbriae are strongly associated with upper urinary tract colonization due to specific binding to α-D-galactopyranosyl-(1-4)-β-D-galactopyranoside receptors in the kidneys. Thus, inhibiting P-fimbrial adhesion may reduce the incidence of UPEC-mediated UTI. E. coli 83972 is an asymptomatic bacteriuria isolate successfully used as a prophylactic agent to prevent UTI in human studies. We constructed a recombinant E. coli 83972 strain displaying a surface-located oligosaccharide P fimbriae receptor mimic that bound to P-fimbriated E. coli producing any of the 3 PapG adhesin variants. The recombinant strain, E. coli 83972:: lgtCE, impaired P fimbriae–mediated adhesion to human erythrocytes and kidney epithelial cells. Additionally, E. coli 83972::lgtCE impaired urine colonization by UPEC in a mouse UTI model, demonstrating its potential as a prophylactic agent to prevent UTI.|
|Rights: ||© The Author 2012.|
|Appears in Collections:||Microbiology and Immunology Publications|
|View citing articles in: ||Google Scholar|
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