Adelaide Research and Scholarship
Schools and Disciplines
School of Molecular and Biomedical Science
Microbiology and Immunology
Microbiology and Immunology publications
Please use this identifier to cite or link to this item:
|Type: ||Journal article|
|Title: ||Escherichia coli 83972 expressing a P fimbriae oligosaccharide receptor mimic impairs adhesion of uropathogenic E. coli|
|Author: ||Watts, Rebecca E.|
Tan, Chee K.
Ulett, Glen C.
Carey, Alison J.
Paton, Adrienne Webster
Paton, James Cleland
Schembri, Mark A.
|Citation: ||Journal of Infectious Diseases, 2012; 206 (8):1242-1249|
|Publisher: ||Oxford University Press on behalf of the Infectious Diseases Society of America|
|Issue Date: ||2012|
|School/Discipline: ||School of Molecular and Biomedical Science : Microbiology and Immunology|
|Rebecca E. Watts, Chee K. Tan, Glen C. Ulett, Alison J. Carey, Makrina Totsika, Adi Idris, Adrienne W. Paton, Renato Morona, James C. Paton, and Mark A. Schembri|
|Abstract: ||Urinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) are a significant health concern, exacerbated by the rapid emergence of multidrug resistant strains refractory to antibiotic treatment. P fimbriae are strongly associated with upper urinary tract colonization due to specific binding to α-D-galactopyranosyl-(1-4)-β-D-galactopyranoside receptors in the kidneys. Thus, inhibiting P-fimbrial adhesion may reduce the incidence of UPEC-mediated UTI. E. coli 83972 is an asymptomatic bacteriuria isolate successfully used as a prophylactic agent to prevent UTI in human studies. We constructed a recombinant E. coli 83972 strain displaying a surface-located oligosaccharide P fimbriae receptor mimic that bound to P-fimbriated E. coli producing any of the 3 PapG adhesin variants. The recombinant strain, E. coli 83972:: lgtCE, impaired P fimbriae–mediated adhesion to human erythrocytes and kidney epithelial cells. Additionally, E. coli 83972::lgtCE impaired urine colonization by UPEC in a mouse UTI model, demonstrating its potential as a prophylactic agent to prevent UTI.|
|Rights: ||© The Author 2012.|
|Appears in Collections:||Microbiology and Immunology publications|
|View citing articles in: ||Google Scholar|
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.