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Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/73588

Type: Journal article
Title: Escherichia coli 83972 expressing a P fimbriae oligosaccharide receptor mimic impairs adhesion of uropathogenic E. coli
Author: Watts, R.
Tan, C.
Ulett, G.
Carey, A.
Totsika, M.
Idris, A.
Paton, A.
Morona, R.
Paton, J.
Schembri, M.
Citation: Journal of Infectious Diseases, 2012; 206:1242-1249
Publisher: Univ Chicago Press
Issue Date: 2012
ISSN: 0022-1899
Statement of
Responsibility: 
Rebecca E. Watts, Chee K. Tan, Glen C. Ulett, Alison J. Carey, Makrina Totsika, Adi Idris, Adrienne W. Paton, Renato Morona, James C. Paton, and Mark A. Schembri
Abstract: Urinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) are a significant health concern, exacerbated by the rapid emergence of multidrug resistant strains refractory to antibiotic treatment. P fimbriae are strongly associated with upper urinary tract colonization due to specific binding to α-D-galactopyranosyl-(1-4)-β-D-galactopyranoside receptors in the kidneys. Thus, inhibiting P-fimbrial adhesion may reduce the incidence of UPEC-mediated UTI. E. coli 83972 is an asymptomatic bacteriuria isolate successfully used as a prophylactic agent to prevent UTI in human studies. We constructed a recombinant E. coli 83972 strain displaying a surface-located oligosaccharide P fimbriae receptor mimic that bound to P-fimbriated E. coli producing any of the 3 PapG adhesin variants. The recombinant strain, E. coli 83972:: lgtCE, impaired P fimbriae–mediated adhesion to human erythrocytes and kidney epithelial cells. Additionally, E. coli 83972::lgtCE impaired urine colonization by UPEC in a mouse UTI model, demonstrating its potential as a prophylactic agent to prevent UTI.
Rights: © The Author 2012.
RMID: 0020122013
DOI: 10.1093/infdis/jis493
Appears in Collections:Microbiology and Immunology publications
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