University of Adelaide Library

Adelaide Research and Scholarship : Schools and Disciplines : School of Agriculture, Food and Wine : Agriculture, Food and Wine publications

Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/74116

Type: Journal article
Title: Functional characterization of the rice SPX-MFS family reveals a key role of OsSPX-MFS1 in controlling phosphate homeostasis in leaves
Author: Wang, C.
Huang, W.
Ying, Y.
Li, S.
Secco, D.
Tyerman, S.
Whelan, J.
Shou, H.
Citation: New Phytologist, 2012; 196(1):139-148
Publisher: Blackwell Publishing Ltd
Issue Date: 2012
ISSN: 0028-646X
1469-8137
Statement of
Responsibility: 
Chuang Wang, Wei Huang, Yinghui Ying, Shuai Li, David Secco, Steve Tyerman, James Whelan and Huixia Shou
Abstract: Proteins possessing the SPX domain are found in several proteins involved in inorganic phosphate (Pi) transport and signalling in yeast and plants. Although the functions of several SPX-domain protein subfamilies have recently been uncovered, the role of the SPX-MFS subfamily is still unclear. Using quantitative RT-PCR analysis, we studied the regulation of SPX-MFS gene expression by the central regulator, OsPHR2 and Pi starvation. The function of OsSPX-MFS1 in Pi homeostasis was analysed using an OsSPX-MFS1 mutant (mfs1) and osa-miR827 overexpression line (miR827-Oe). Finally, heterologous complementation of a yeast mutant impaired in Pi transporter was used to assess the capacity of OsSPX-MFS1 to transport Pi. Transcript analyses revealed that members of the SPX-MFS family were mainly expressed in the shoots, with OsSPX-MFS1 and OsSPX-MFS3 being suppressed by Pi deficiency, while OsSPX-MFS2 was induced. Mutation in OsSPX-MFS1 (mfs1) and overexpression of the upstream miR827 (miR827-Oe) plants impaired Pi homeostasis in the leaves. In addition, studies in yeast revealed that OsSPX-MFS1 may be involved in Pi transport. The results suggest that OsSPX-MFS1 is a key player in maintaining Pi homeostasis in the leaves, potentially acting as a Pi transporter.
Keywords: Homeostasis; miRNA; OsPHR2; phosphate; rice; SPX; transporter
Rights: © 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
RMID: 0020121797
DOI: 10.1111/j.1469-8137.2012.04227.x
Appears in Collections:Agriculture, Food and Wine publications
View citing articles in: Web of Science
Google Scholar
Scopus

There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

© 2008 The University of Adelaide
library@adelaide.edu.au
CRICOS Provider Number 00123M
Service Charter | Copyright | Privacy | Disclaimer