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Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/74249

Type: Journal article
Title: Cytosine methylation regulates oviposition in the pathogenic blood fluke Schistosoma mansoni
Author: Geyer, Kathrin K.
Rodriguez Lopez, Carlos Marcelino
Chalmers, Iain W.
Munshi, Sabrina E.
Truscott, Martha
Heald, James
Wilkinson, Michael James
Hoffmann, Karl F.
Citation: Nature Communications, 2011; 2:424
Publisher: Nature Publishing Group
Issue Date: 2011
ISSN: 2041-1723
School/Discipline: School of Agriculture, Food and Wine
Statement of
Responsibility: 
Kathrin K. Geyer, Carlos M. Rodríguez López, Iain W. Chalmers, Sabrina E. Munshi, Martha Truscott, James Heald, Mike J. Wilkinson and Karl F. Hoffmann
Abstract: Similar to other metazoan pathogens, Schistosoma mansoni undergoes transcriptional and developmental regulation during its complex lifecycle and host interactions. DNA methylation as a mechanism to control these processes has, to date, been discounted in this parasite. Here we show the first evidence for cytosine methylation in the S. mansoni genome. Transcriptional coregulation of novel DNA methyltransferase (SmDnmt2) and methyl-CpG-binding domain proteins mirrors the detection of cytosine methylation abundance and implicates the presence of a functional DNA methylation machinery. Genome losses in cytosine methylation upon SmDnmt2 silencing and the identification of a hypermethylated, repetitive intron within a predicted forkhead gene confirm this assertion. Importantly, disruption of egg production and egg maturation by 5-azacytidine establishes an essential role for 5-methylcytosine in this parasite. These findings provide the first functional confirmation for this epigenetic modification in any worm species and link the cytosine methylation machinery to platyhelminth oviposition processes.
Rights: © 2011 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.
RMID: 0020118817
DOI: 10.1038/ncomms1433
Appears in Collections:Agriculture, Food and Wine publications
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