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Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/74258

Type: Journal article
Title: Identification of mediator complex 26 (Crsp7) gametologs on platypus X1 and Y5 sex chromosomes: a candidate testis-determining gene in monotremes?
Author: Tsend-Ayush, Enkhjargal
Kortschak, Robert Daniel
Bernard, Pascal
Lim, Shu Ly
Ryan, Janelle
Rosenkranz, Ruben
Borodina, Tatiana
Dohm, Juliane C.
Himmelbauer, Heinz
Harley, Vincent
Grutzner, Frank
Citation: Chromosome Research, 2012; 20(1):127-138
Publisher: Springer
Issue Date: 2012
ISSN: 0967-3849
School/Discipline: School of Molecular and Biomedical Science
Statement of
Responsibility: 
Enkhjargal Tsend-Ayush, R. Daniel Kortschak, Pascal Bernard, Shu Ly Lim, Janelle Ryan, Ruben Rosenkranz, Tatiana Borodina, Juliane C. Dohm, Heinz Himmelbauer, Vincent R. Harley and Frank Grützner
Abstract: The basal lineage of monotremes features an extraordinarily complex sex chromosome system which has provided novel insights into the evolution of mammalian sex chromosomes. Recently, sequence information from autosomes, X chromosomes, and XY-shared pseudoautosomal regions has become available. However, no gene has so far been described on any of the Y chromosome-specific regions. We analyzed sequences derived from Y-specific BAC clones to identify genes with potentially malespecific function. Here, we report the identification and characterization of the mediator complex protein gametologs on platypus Y5 (Crspy). We also identified the X-chromosomal copy which unexpectedly maps to X1 (Crspx). Sequence comparison shows extensive divergence between the X and Y copy, but we found no significant positive selection on either gametolog. Expression analysis shows widespread expression of Crspx. Crspy is expressed exclusively in males with particularly strong expression in testis and kidney. Reporter gene assays to investigate whether Crspx/y can act on the recently discovered mouse Sox9 testis-specific enhancer element did reveal a modest effect together with mouse Sox9+Sf1, but showed overall no significant upregulation of the reporter gene. This is the first report of a differentiated functional male-specific gene on platypus Y chromosomes, providing new insights into sex chromosome evolution and a candidate gene for male-specific function in monotremes.
Keywords: Evolution; sex chromosomes; Y chromosomes; mediator complex; sex determination; mammals; Tachyglossus aculeatus; Ornithorhynchus anatinus
Rights: © Springer Science+Business Media B.V. 2011
RMID: 0020117357
DOI: 10.1007/s10577-011-9270-z
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Molecular and Biomedical Science publications
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