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Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/74618

Type: Journal article
Title: Research resource: interplay between the genomic and transcriptional networks of androgen receptor and estrogen receptor α in luminal breast cancer cells
Other Titles: Research resource: interplay between the genomic and transcriptional networks of androgen receptor and estrogen receptor alpha in luminal breast cancer cells
Author: Need, E.
Selth, L.
Harris, T.
Birrell, S.
Tilley, W.
Buchanan, G.
Citation: Molecular Endocrinology, 2012; 26(11):1941-1952
Publisher: Endocrine Soc
Issue Date: 2012
ISSN: 0888-8809
1944-9917
Statement of
Responsibility: 
Eleanor F. Need, Luke A. Selth, Tiffany J. Harris, Stephen N. Birrell, Wayne D. Tilley and Grant Buchanan
Abstract: The cellular response to circulating sex steroids is more than the sum of individual hormone actions, instead representing an interplay between activities of the evolutionarily related steroid hormone receptors. An example of this interaction is in breast cancer, where the risk of dying from estrogen receptor-_ (ER_)-positive disease decreases approximately 4-fold when androgen receptor (AR) expression is high. In this study, we used chromatin immunoprecipitation sequencing (ChIP-seq) and microarray expression profiling to investigate the genomic and transcriptional cross talk between AR and ER_ signaling in a luminal breast cancer cell line model, ZR-75–1. Expression profiling demonstrated reciprocal interference between 5_-dihydrotestosterone (DHT)- and 17_-estradiol (E2)-induced transcriptional programs. Specifically, regulation of 26% of E2 and 15% of DHT target genes was significantly affected by cotreatment with the other hormone, in the majority of cases (78–83%) antagonistically. Pathway analysis suggested that DHT cotreatment, for example, depleted E2-regulated pathways in cell survival and proliferation. ChIP-seq identified substantial overlap between the steroid receptor cistromes in ZR-75–1 cells, with 10–13% of AR- and ER_-binding sites located within 10 kb of the other receptor. Enrichment of androgen response elements in ER_-binding sites and vice versa was revealed by motif analysis, and AR-binding sites were enriched about E2-responsive genes affected by DHT cotreatment. Targeted ChIP and expression analysis revealed locus-specific outcomes when AR and ER_ bind to the same DNA region. This work provides the first cistrome data for two steroid receptors in the same cell, insight into the antagonistic interplay between estrogens and androgens in luminal breast cancer, and an important resource for future work aimed at evaluating interrelated steroid receptors in different cellular systems.
Keywords: NURSA molecule pages; nuclear receptors
Rights: Copyright © by The Endocrine Society
RMID: 0020122893
DOI: 10.1210/me.2011-1314
Appears in Collections:Medicine publications
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