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https://hdl.handle.net/2440/75045
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Type: | Journal article |
Title: | Regular care and maintenance of a Zebrafish (Danio rerio) laboratory: an introduction |
Author: | Avdesh, A. Chen, M. Martin-Iverson, M. Mondal, A. Ong, D. Rainey-Smith, S. Taddei, K. Lardelli, M. Groth, D. Verdile, G. Martins, R. |
Citation: | Journal of Visualized Experiments, 2012; (69):1-8 |
Publisher: | Journal of Visualized Experiments |
Issue Date: | 2012 |
ISSN: | 1940-087X 1940-087X |
Statement of Responsibility: | Avdesh Avdesh, Mengqi Chen, Mathew T. Martin-Iverson, Alinda Mondal, Daniel Ong, Stephanie Rainey-Smith, Kevin Taddei, Michael Lardelli, David M. Groth, Giuseppe Verdile and Ralph N. Martins |
Abstract: | This protocol describes regular care and maintenance of a zebrafish laboratory. Zebrafish are now gaining popularity in genetics, pharmacological and behavioural research. As a vertebrate, zebrafish share considerable genetic sequence similarity with humans and are being used as an animal model for various human disease conditions. The advantages of zebrafish in comparison to other common vertebrate models include high fecundity, low maintenance cost, transparent embryos, and rapid development. Due to the spur of interest in zebrafish research, the need to establish and maintain a productive zebrafish housing facility is also increasing. Although literature is available for the maintenance of a zebrafish laboratory, a concise video protocol is lacking. This video illustrates the protocol for regular housing, feeding, breeding and raising of zebrafish larvae. This process will help researchers to understand the natural behaviour and optimal conditions of zebrafish husbandry and hence troubleshoot experimental issues that originate from the fish husbandry conditions. This protocol will be of immense help to researchers planning to establish a zebrafish laboratory, and also to graduate students who are intending to use zebrafish as an animal model. |
Keywords: | Animals Animals, Laboratory Zebrafish Laboratory Animal Science Animal Welfare Animal Husbandry Female Male |
Rights: | Copyright © JoVE 2006-2013. All Rights Reserved. |
DOI: | 10.3791/4196 |
Published version: | http://dx.doi.org/10.3791/4196 |
Appears in Collections: | Aurora harvest 4 Molecular and Biomedical Science publications |
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