Isolation and characterization of 19 new microsatellites for European grayling, Thymallus thymallus (Linnaeus, 1758), and their cross-amplification in four other salmonid species

Abstract

The European grayling (Thymallus thymallus) is a locally endangered and economically important freshwater salmonid fish that is distributed across a large part of Europe. Here we report 19 polymorphic microsatellites, doubling the number of available markers. Of those, 17 loci amplified in the closely related Arctic grayling (T. arcticus). Seven, six and ten amplified in the more distantly related salmonids Atlantic salmon (Salmo salar), Arctic charr (Salvelinus alpinus) and brown trout (Salmo trutta), respectively.