Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/85587
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Type: | Journal article |
Title: | Regulation of fatty acid oxidation in mouse cumulus-oocyte complexes during maturation and modulation by PPAR agonists |
Author: | Dunning, K. Anastasi, M. Zhang, V. Russell, D. Robker, R. |
Citation: | PLoS One, 2014; 9(2):e87327-1-e87327-11 |
Publisher: | Public Library of Science |
Issue Date: | 2014 |
ISSN: | 1932-6203 1932-6203 |
Editor: | Lim, H.J. |
Statement of Responsibility: | Kylie R. Dunning, Marie R. Anastasi, Voueleng J. Zhang, Darryl L. Russell, Rebecca L. Robker |
Abstract: | Fatty acid oxidation is an important energy source for the oocyte; however, little is known about how this metabolic pathway is regulated in cumulus-oocyte complexes. Analysis of genes involved in fatty acid oxidation showed that many are regulated by the luteinizing hormone surge during in vivo maturation, including acyl-CoA synthetases, carnitine transporters, acyl-CoA dehydrogenases and acetyl-CoA transferase, but that many are dysregulated when cumulus-oocyte complexes are matured under in vitro maturation conditions using follicle stimulating hormone and epidermal growth factor. Fatty acid oxidation, measured as production of 3H2O from [3H]palmitic acid, occurs in mouse cumulus-oocyte complexes in response to the luteinizing hormone surge but is significantly reduced in cumulus-oocyte complexes matured in vitro. Thus we sought to determine whether fatty acid oxidation in cumulus-oocyte complexes could be modulated during in vitro maturation by lipid metabolism regulators, namely peroxisome proliferator activated receptor (PPAR) agonists bezafibrate and rosiglitazone. Bezafibrate showed no effect with increasing dose, while rosiglitazone dose dependently inhibited fatty acid oxidation in cumulus-oocyte complexes during in vitro maturation. To determine the impact of rosiglitazone on oocyte developmental competence, cumulus-oocyte complexes were treated with rosiglitazone during in vitro maturation and gene expression, oocyte mitochondrial activity and embryo development following in vitro fertilization were assessed. Rosiglitazone restored Acsl1, Cpt1b and Acaa2 levels in cumulus-oocyte complexes and increased oocyte mitochondrial membrane potential yet resulted in significantly fewer embryos reaching the morula and hatching blastocyst stages. Thus fatty acid oxidation is increased in cumulus-oocyte complexes matured in vivo and deficient during in vitro maturation, a known model of poor oocyte quality. That rosiglitazone further decreased fatty acid oxidation during in vitro maturation and resulted in poor embryo development points to the developmental importance of fatty acid oxidation and the need for it to be optimized during in vitro maturation to improve this reproductive technology. |
Keywords: | Oocytes Animals Mice, Inbred CBA Bezafibrate Thiazolidinediones Hormones Epidermal Growth Factor Follicle Stimulating Hormone Fatty Acids Peroxisome Proliferator-Activated Receptors Hypoglycemic Agents Gene Expression Regulation Oxidation-Reduction Female Lipid Metabolism Cumulus Cells Hypolipidemic Agents Rosiglitazone |
Rights: | © 2014 Dunning et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
DOI: | 10.1371/journal.pone.0087327 |
Grant ID: | http://purl.org/au-research/grants/arc/DE120100304 |
Published version: | http://dx.doi.org/10.1371/journal.pone.0087327 |
Appears in Collections: | Aurora harvest 2 Obstetrics and Gynaecology publications |
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File | Description | Size | Format | |
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hdl_85587.pdf | Published version | 3.77 MB | Adobe PDF | View/Open |
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