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https://hdl.handle.net/2440/86450
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Type: | Journal article |
Title: | Development, characterisation and cross-species amplification of 16 novel microsatellite markers for the endangered Black-throated Finch (Poephila cincta) in Australia |
Author: | Tang, L. Smith-Keune, C. Gardner, M. Hardesty, B. |
Citation: | Conservation Genetics Resources, 2014; 6(1):143-146 |
Publisher: | Springer Netherlands |
Issue Date: | 2014 |
ISSN: | 1877-7252 1877-7260 |
Statement of Responsibility: | Lei Tang, Carolyn Smith-Keune, Michael G. Gardner, Britta Denise Hardesty |
Abstract: | The Black-throated Finch (Southern) (Poephila cincta cincta) is threatened by the substantial landscape changes in northern Australia. We developed 16 polymorphic microsatellite markers using 454-shotgun whole-genome sequencing technology. We identified an average of 4.7 alleles per locus based on 63 wild caught individuals from Townsville, Queensland. Thirteen and 9 markers were also successfully cross-amplified in two confamilial species, the Double-barred Finch (Taeniopygia bichenovii) and the chestnut-breasted Mannikin (Lonchura castaneothorax) with 11 and 5 were polymorphic, respectively. These markers will help understand the population genetic structure of the endangered Black-throated Finch and determine genetic consequences of landscape changes for the species. |
Keywords: | Black-throated Finch; Poephila cincta; Microsatellite; Conservation genetics; Next generation sequencing |
Rights: | © Springer Science+Business Media Dordrecht 2013 |
DOI: | 10.1007/s12686-013-0028-0 |
Published version: | http://dx.doi.org/10.1007/s12686-013-0028-0 |
Appears in Collections: | Aurora harvest 2 Earth and Environmental Sciences publications |
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