Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/100122
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Type: Journal article
Title: Transcranial magnetic stimulation of human adult stem cells in the mammalian brain
Author: Kremer, K.
Smith, A.
Sandeman, L.
Inglis, J.
Ridding, M.
Koblar, S.
Citation: Frontiers in Neural Circuits, 2016; 10(MAR):17-1-17-9
Publisher: Frontiers Media SA
Issue Date: 2016
ISSN: 1662-5110
1662-5110
Statement of
Responsibility: 
Karlea L. Kremer, Ashleigh E. Smith, Lauren Sandeman, Joshua M. Inglis, Michael C. Ridding, and Simon A. Koblar
Abstract: Introduction: The burden of stroke on the community is growing, and therefore, so is the need for a therapy to overcome the disability following stroke. Cellular-based therapies are being actively investigated at a pre-clinical and clinical level. Studies have reported the beneficial effects of exogenous stem cell implantation, however, these benefits are also associated with limited survival of implanted stem cells. This exploratory study investigated the use of transcranial magnetic stimulation (TMS) as a complementary therapy to increase stem cell survival following implantation of human dental pulp stem cells (DPSC) in the rodent cortex. Methods: Sprague-Dawley rats were anesthetized and injected with 6 x 10⁵ DPSC or control media via an intracranial injection, and then received real TMS (TMS₀.₂ Hz) or sham TMS (TMSsham) every 2nd day beginning on day 3 post DPSC injection for 2 weeks. Brain sections were analyzed for the survival, migration and differentiation characteristics of the implanted cells. Results: In animals treated with DPSC and TMS₀.₂ Hz there were significantly less implanted DPSC and those that survived remained in the original cerebral hemisphere compared to animals that received TMSsham. The surviving implanted DPSC in TMS₀.₂ Hz were also found to express the apoptotic marker Caspase-3. Conclusions: We suggest that TMS at this intensity may cause an increase in glutamate levels, which promotes an unfavorable environment for stem cell implantation, proliferation and differentiation. It should be noted that only one paradigm of TMS was tested as this was conducted as a exploratory study, and further TMS paradigms should be investigated in the future.
Keywords: Stroke; dental pulp stem cells; transcranial magnetic stimulation; glutamate; rat
Rights: Copyright © 2016 Kremer, Smith, Sandeman, Inglis, Ridding and Koblar. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
RMID: 0030046449
DOI: 10.3389/fncir.2016.00017
Grant ID: http://purl.org/au-research/grants/nhmrc/1097397
Appears in Collections:Medicine publications

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