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https://hdl.handle.net/2440/100161
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Type: | Journal article |
Title: | Intranasal vaccination with γ -irradiated Streptococcus pneumoniae whole-cell vaccine provides serotype-independent protection mediated by B-cells and innate IL-17 responses |
Other Titles: | Intranasal vaccination with gamma -irradiated Streptococcus pneumoniae whole-cell vaccine provides serotype-independent protection mediated by B-cells and innate IL-17 responses |
Author: | Babb, R. Chen, A. Hirst, T. Kara, E. McColl, S. Ogunniyi, A. Paton, J. Alsharifi, M. |
Citation: | Clinical Science, 2016; 130(9):697-710 |
Publisher: | Portland Press |
Issue Date: | 2016 |
ISSN: | 0143-5221 1470-8736 |
Statement of Responsibility: | Rachelle Babb, Austen Chen, Timothy R. Hirst, Ervin E. Kara, Shaun R. McColl, Abiodun D. Ogunniyi, James C. Paton, and Mohammed Alsharifi |
Abstract: | Generating a pneumococcal vaccine that is serotype independent and cost effective remains a global challenge. γ-Irradiation has been used widely to sterilize biological products. It can also be utilized as an inactivation technique to generate whole-cell bacterial and viral vaccines with minimal impact on pathogen structure and antigenic determinants. In the present study, we utilized γ-irradiation to inactivate an un-encapsulated Streptococcus pneumoniae strain Rx1 with an unmarked deletion of the autolysin gene lytA and with the pneumolysin gene ply replaced with an allele encoding a non-toxic pneumolysoid (PdT) (designated γ-PN vaccine). Intranasal vaccination of C57BL/6 mice with γ-PN was shown to elicit serotype-independent protection in lethal challenge models of pneumococcal pneumonia and sepsis. Vaccine efficacy was shown to be reliant on B-cells and interleukin (IL)-17A responses. Interestingly, immunization promoted IL-17 production by innate cells not T helper 17 (Th17) cells. These data are the first to report the development of a non-adjuvanted intranasal γ-irradiated pneumococcal vaccine that generates effective serotype-independent protection, which is mediated by both humoral and innate IL-17 responses. |
Keywords: | Protective immunity; serotype independent; Streptococcus pneumoniae; vaccine |
Rights: | © 2016 Authors; published by Portland Press Limited |
DOI: | 10.1042/CS20150699 |
Grant ID: | http://purl.org/au-research/grants/arc/LP120200244 NHMRC |
Published version: | http://dx.doi.org/10.1042/cs20150699 |
Appears in Collections: | Aurora harvest 7 Molecular and Biomedical Science publications |
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