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Type: Journal article
Title: RootGraph: a graphic optimization tool for automated image analysis of plant roots
Author: Cai, J.
Zeng, Z.
Connor, J.
Huang, C.
Melino, V.
Kumar, P.
Miklavcic, S.
Citation: Journal of Experimental Botany, 2015; 66(21):6551-6562
Publisher: Oxford University Press
Issue Date: 2015
ISSN: 0022-0957
Statement of
Jinhai Cai, Zhanghui Zeng, Jason N. Connor, Chun Yuan Huang, Vanessa Melino, Pankaj Kumar and Stanley J. Miklavcic
Abstract: This paper outlines a numerical scheme for accurate, detailed, and high-throughput image analysis of plant roots. In contrast to existing root image analysis tools that focus on root system-average traits, a novel, fully automated and robust approach for the detailed characterization of root traits, based on a graph optimization process is presented. The scheme, firstly, distinguishes primary roots from lateral roots and, secondly, quantifies a broad spectrum of root traits for each identified primary and lateral root. Thirdly, it associates lateral roots and their properties with the specific primary root from which the laterals emerge. The performance of this approach was evaluated through comparisons with other automated and semi-automated software solutions as well as against results based on manual measurements. The comparisons and subsequent application of the algorithm to an array of experimental data demonstrate that this method outperforms existing methods in terms of accuracy, robustness, and the ability to process root images under high-throughput conditions.
Keywords: 2D; fully automated; graphic optimization; high throughput; image analysis; root network analysis; root phenotyping; wheat and barley
Rights: © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.
RMID: 0030033228
DOI: 10.1093/jxb/erv359
Appears in Collections:Environment Institute publications

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