Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/10154
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Type: Journal article
Title: Methionine derivatives diminish sulphide damage to colonocytes - implications for ulcerative colitis.
Author: Roediger, W.
Babidge, W.
Millard, S.
Citation: Gut, 1996; 39(1):77-81
Publisher: B M J PUBLISHING GROUP
Issue Date: 1996
ISSN: 0017-5749
1468-3288
Abstract: BACKGROUND:Bacterial production of anionic sulphide is increased in the colon of ulcerative colitis and sulphides can cause metabolic damage to colonocytes. AIMS:To assess the reversal of the damaging effect of sulphide to isolated colonocytes by methionine and methionine derivatives. METHODS AND SUBJECTS:Isolated colonocytes were prepared from rat colons and 12 human colectomy specimens. In cell suspensions 14CO2/acetoacetate generation was measured from [1-14C]-butyrate (5.0 mmol/l) in the presence of 0-2.0 mmol/l sodium hydrogen sulphide. The effect of 5.0 mmol/l L-methionine, S-adenosylmethionine 1,4 butane disulphonate and DL-methionine-S-methylsulphonium chloride on sulphide inhibited oxidation was observed. RESULTS:In rat colonocytes sodium hydrogen sulphide dose dependently reduced oxidative metabolite formation from n-butyrate, an action reversed in order of efficacy by S-adenosylmethionine 1,4 butane disulphonate > DLmethionine-S-methyl-sulphonium chloride > L-methionine. In human colonocytes S-adenosylmethionine 1,4 butane disulphonate most significantly improved 14CO2 production (p = < 0.005) suppressed by sodium hydrogen sulphide. CONCLUSION:Sulphide toxicity in colonocytes is reversible by methyl donors. The efficiency of sulphide detoxification may be an important factor in the pathogenesis and treatment of ulcerative colitis for which S-adenosylmethionine 1,4 butane disulphonate may be of therapeutic value.
Keywords: Colon
Cells, Cultured
Animals
Humans
Rats
Rats, Sprague-Dawley
Colitis, Ulcerative
Carbon Dioxide
Sulfides
Carbon Radioisotopes
Acetamides
Butyric Acid
Butyrates
Vitamin U
Methionine
S-Adenosylmethionine
Scintillation Counting
Male
DOI: 10.1136/gut.39.1.77
Appears in Collections:Aurora harvest 7
Surgery publications

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