Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/101766
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Drosophila Pkaap regulates Rab4/Rab11-dependent traffic and Rab11 exocytosis of innate immune cargo
Author: Sorvina, A.
Shandala, T.
Brooks, D.
Citation: Biology Open, 2016; 5(6):678-688
Publisher: Company of Biologists
Issue Date: 2016
ISSN: 2046-6390
2046-6390
Statement of
Responsibility: 
Alexandra Sorvina, Tetyana Shandala and Douglas A. Brooks
Abstract: The secretion of immune-mediators is a critical step in the host innate immune response to pathogen invasion, and Rab GTPases have an important role in the regulation of this process. Rab4/Rab11 recycling endosomes are involved in the sorting of immune-mediators into specialist Rab11 vesicles that can traffic this cargo to the plasma membrane; however, how this sequential delivery process is regulated has yet to be fully defined. Here, we report that Drosophila Pkaap, an orthologue of the human dual-specific A-kinase-anchoring protein 2 or D-AKAP2 (also called AKAP10), appeared to have a nucleotide-dependent localisation to Rab4 and Rab11 endosomes. RNAi silencing of pkaap altered Rab4/Rab11 recycling endosome morphology, suggesting that Pkaap functions in cargo sorting and delivery in the secretory pathway. The depletion of pkaap also had a direct effect on Rab11 vesicle exocytosis and the secretion of the antimicrobial peptide Drosomycin at the plasma membrane. We propose that Pkaap has a dual role in antimicrobial peptide traffic and exocytosis, making it an essential component for the secretion of inflammatory mediators and the defence of the host against pathogens.
Keywords: Antimicrobial peptide Drosomycin
drosophila
endosomes
innate immunity
Pkaap
Rab11
Rab4
Rights: © 2016. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
DOI: 10.1242/bio.016642
Grant ID: http://purl.org/au-research/grants/nhmrc/631915
Appears in Collections:Aurora harvest 7
Medicine publications

Files in This Item:
File Description SizeFormat 
hdl_101766.pdfPublished Version8.44 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.