Please use this identifier to cite or link to this item:
Type: Journal article
Title: Lack of interleukin-2 (IL-2) cytokine expression despite IL-2mRNA transcription in tumour infiltrating lymphocytes in primary human breast carcinoma : selective expression of early activation markers
Author: Coventry, B.
Weeks, S.
Heckford, S.
Sykes, P.
Skinner, J.
Bradley, J.
Citation: Journal of Immunology, 1996; 156(9):3486-3492
Issue Date: 1996
ISSN: 0022-1767
Abstract: Tumor-infiltrating lymphocytes (TIL) are found in most human infiltrating ductal breast carcinomas. In studies of other tumors, TIL were capable of activation by IL-2, both in vitro and in vivo, to produce selective tumor cytolysis. Specific TIL-mediated tumor cytolysis in human breast tumors has recently been reported. The large numbers of TIL within human breast cancers imply that an immune response is occurring, since many of these cells express HLA class II as a late activation marker. However, the degree of early activation of the native TIL in breast tumors has not been fully investigated. Early activation markers CD69, CD43, and CD38 together with the IL-2R (CD25) and IL-2 cytokine were examined using mAbs and tissue section immunohistology. In situ hybridization was used to detect IL-2 mRNA (IL-2 mRNA) in parallel with immunohistochemical localization of IL-2. The results revealed the expression of CD69, CD43, and CD38, but markedly low CD25 (IL-2R) and IL-2 protein expression by the TIL. This strongly indicates that the TIL are an activated population of T cells that shows a deficiency in IL-2 protein and IL-2R expression despite adequate levels of IL-2 mRNA. The mechanism for apparent inhibition of IL-2 production and IL-2R expression in the presence of IL-2 mRNA is currently unclear; however, this may explain the relative anergic state of native TIL.
Keywords: Lymphocytes, Tumor-Infiltrating
Breast Neoplasms
Receptors, Interleukin-2
RNA, Messenger
Antigens, Differentiation, T-Lymphocyte
In Situ Hybridization
Prospective Studies
Transcription, Genetic
Appears in Collections:Aurora harvest 7
Surgery publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.