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|Title:||COMMD1 is linked to the WASH complex and regulates endosomal trafficking of the copper transporter ATP7A|
Van De Sluis, B.
|Citation:||Molecular Biology of the Cell, 2015; 26(1):91-103|
|Publisher:||American Society for Cell Biology|
|Christine A. Phillips-Krawczak, Amika Singla, Petro Starokadomskyy, Zhihui Deng, Douglas G. Osbornea, Haiying Li, Christopher J. Dick, Timothy S. Gomez, Megan Koenecke, Jin-San Zhang, Haiming Dai, Luis F. Sifuentes-Dominguez, Linda N. Geng, Scott H. Kaufmann, Marco Y. Hein, Mathew Wallis, Julie McGaughran, Jozef Gecz, Bart van de Sluis, Daniel D. Billadeau and Ezra Burstein|
|Abstract:||COMMD1 deficiency results in defective copper homeostasis, but the mechanism for this has remained elusive. Here we report that COMMD1 is directly linked to early endosomes through its interaction with a protein complex containing CCDC22, CCDC93, and C16orf62. This COMMD/CCDC22/CCDC93 (CCC) complex interacts with the multisubunit WASH complex, an evolutionarily conserved system, which is required for endosomal deposition of F-actin and cargo trafficking in conjunction with the retromer. Interactions between the WASH complex subunit FAM21, and the carboxyl-terminal ends of CCDC22 and CCDC93 are responsible for CCC complex recruitment to endosomes. We show that depletion of CCC complex components leads to lack of copper-dependent movement of the copper transporter ATP7A from endosomes, resulting in intracellular copper accumulation and modest alterations in copper homeostasis in humans with CCDC22 mutations. This work provides a mechanistic explanation for the role of COMMD1 in copper homeostasis and uncovers additional genes involved in the regulation of copper transporter recycling.|
|Rights:||© 2015 Phillips-Krawczak, Singla, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/ licenses/by-nc-sa/3.0).|
|Appears in Collections:||Aurora harvest 3|
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