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Type: Journal article
Title: The LOSS OF APOMEIOSIS (LOA) locus in Hieracium praealtum can function independently of the associated large-scale repetitive chromosomal structure
Author: Kotani, Y.
Henderson, S.
Suzuki, G.
Johnson, S.
Okada, T.
Siddons, H.
Mukai, Y.
Koltunow, A.
Citation: New Phytologist, 2014; 201(3):973-981
Publisher: Wiley-Blackwell
Issue Date: 2014
ISSN: 0028-646X
Statement of
Yoshiko Kotani, Steven T. Henderson, Go Suzuki, Susan D. Johnson, Takashi Okada, Hayley Siddons, Yasuhiko Mukai and Anna M. G. Koltunow
Abstract: Apomixis or asexual seed formation in Hieracium praealtum (Asteraceae) is controlled by two independent dominant loci. One of these, the LOSS OF APOMEIOSIS (LOA) locus, controls apomixis initiation, mitotic embryo sac formation (apospory) and suppression of the sexual pathway. The LOA locus is found near the end of a hemizygous chromosome surrounded by extensive repeats extending along the chromosome arm. Similar apomixis-carrying chromosome structures have been found in some apomictic grasses, suggesting that the extensive repetitive sequences may be functionally relevant to apomixis. Fluorescence in situ hybridization (FISH) was used to examine chromosomes of apomeiosis deletion mutants and rare recombinants in the critical LOA region arising from a cross between sexual Hieracium pilosella and apomictic H. praealtum. The combined analyses of aposporous and nonaposporous recombinant progeny and chromosomal karyotypes were used to determine that the functional LOA locus can be genetically separated from the very extensive repeat regions found on the LOA-carrying chromosome. The large-scale repetitive sequences associated with the LOA locus in H. praealtum are not essential for apospory or suppression of sexual megasporogenesis (female meiosis).
Keywords: Apomeiosis; apomixis; apospory; chromosome structure; fluorescence in situ hybridization (FISH); Hieracium
Rights: © 2013 CSIRO New Phytologist © 2013 New Phytologist Trust
RMID: 0030015660
DOI: 10.1111/nph.12574
Appears in Collections:Agriculture, Food and Wine publications

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