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Type: Journal article
Title: A primer extension denaturing high-performance liquid chromatography method for the identification of three ABCC2 genetic polymorphisms
Author: Westley, I.
Coller, J.
Ward, M.
Evans, A.
Morris, R.
Sallustio, B.
Citation: Journal of Liquid Chromatography and Related Technologies, 2014; 37(9):1249-1256
Publisher: Taylor and Francis
Issue Date: 2014
ISSN: 1082-6076
Statement of
Ian S. Westley, Janet K. Coller, Michael B. Ward, Allan M. Evans, Raymond G. Morris, and Benedetta C. Sallustio
Abstract: Background: A number of single nucleotide polymorphisms have been described in the ABCC2 gene that alters drug disposition. The aim of the study was to develop a primer extension denaturing high-performance liquid chromatography (PE-dHPLC) assay to determine three common variants of the ABCC2 gene in the promoter region (−24C>T), exon 10 (1249G>A), and exon 28 (3972C>T). Methods: Polymerase chain reactions (PCRs) were used to isolate the area of interest in the ABCC2 gene. A comparison of the PCR product was performed between sequencing and dHPLC. Results: A 100% identity match was achieved between groups and allowed for a quick and accurate method to determine three single nucleotide polymorphisms in a single extension reaction. This assay is the first of its type to determine three ABCC2 variants by dHPLC
Keywords: ABCC2; genotype; polymerase chain reaction; polymorphisms; PE-dHPLC; single nudeotide polymorphism
Rights: Copyright © Taylor & Francis Group, LLC
DOI: 10.1080/10826076.2013.789796
Appears in Collections:Aurora harvest 7
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