Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/102270
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Type: Journal article
Title: Engineering DN hydrogels from regenerated silk fibroin and poly(N-vinylcaprolactam)
Author: Whittaker, J.
Dutta, N.
Zannettino, A.
Choudhury, N.
Citation: Journal of Materials Chemistry B, 2016; 4(33):5519-5533
Publisher: Royal Society of Chemistry
Issue Date: 2016
ISSN: 2050-750X
2050-7518
Statement of
Responsibility: 
Jasmin L. Whittaker, Naba K. Dutta, Andrew Zannettino and Namita Roy Choudhury
Abstract: The development of novel hydrogels that possess adequate elasticity and toughness to withstand mechanically active environments, along with being biocompatible, remains a significant challenge in the design of materials for tissue engineering applications. In this study, a family of regenerated silk fibroin (RSF) based double network (DN) hydrogels were fabricated for the first time using a rapid one-pot method. The DN hydrogels combine the rigid covalently crosslinked RSF with the softer poly(N-vinylcaprolactam) (PVCL) through strong physical interactions. The formation of these DN hydrogels resulted in an improvement of the water uptake capacity, elasticity and toughness compared to the individual RSF hydrogel. The elasticity of the RSF/PVCL DN hydrogels was closer to that of native cartilage, which makes them promising materials for cartilage regeneration applications. An in vitro study on adhesion, proliferation and differentiation of a mouse pre-chondrocyte cell line (ATDC5) conducted using microscopic analysis, a cell proliferation assay and RT-PCR confirmed the cells cultured on the less stiff hydrogels demonstrated the most favourable chondrogenic response. Thus, this study demonstrates the potential of RSF-based hybrid hydrogels for cartilage tissue engineering applications.
Description: First published online 05 Jul 2016
Rights: This journal is © The Royal Society of Chemistry 2016
RMID: 0030056363
DOI: 10.1039/c6tb01055e
Grant ID: http://purl.org/au-research/grants/arc/DP1092678
http://purl.org/au-research/grants/arc/DP120103537
Appears in Collections:Medical Sciences publications

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