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dc.contributor.authorTuck, A.-
dc.contributor.authorRobker, R.-
dc.contributor.authorNorman, R.-
dc.contributor.authorTilley, W.-
dc.contributor.authorHickey, T.-
dc.identifier.citationJournal of Ovarian Research, 2015; 8(1):31-1-31-12-
dc.description.abstractThe c-kit/kit ligand (KITL) signalling axis is an essential component of ovarian folliculogenesis in mammals, but little is known about expression and localisation of its key components in the ovaries of reproductive age women. This study aimed to characterise mRNA expression of c-kit and KITL isoforms and the localisation of c-kit and KITL proteins in adult human premenopausal ovaries.This study utilised granulosa cells obtained from the preovulatory follicles of women undergoing assisted reproduction, pieces of ovarian tissue obtained from premenopausal women undergoing gynaecological surgeries and archival paraffin-embedded premenopausal ovarian tissues. Methodology included PCR for gene expression and Western blot or immunohistochemistry for protein expression.Both c-kit mRNA isoforms, known as GNNK+ and GNNK-, were detected in human ovarian cortex, while KITL protein isoforms (KITL1 and KITL2) were present in ovarian cortex and human granulosa cells. Immunohistochemistry showed expression of KITL and c-kit protein in multiple cell types within follicles throughout development, from primordial follicles to large antral follicles, in addition to atretic follicles. Oocytes of all follicle stages expressed c-kit protein exclusively. Interestingly, unlike animal models, expression of both proteins displayed a less cell-type specific distribution with immunostaining present in granulosa, theca and stromal cells, suggesting that autocrine signalling occurs within the human ovary.The results of this study indicate that c-kit/KITL signalling also occurs in the human ovary, as established in various animal models, and may involve previously unknown autocrine signalling.-
dc.description.statementofresponsibilityAstrud R Tuck, Rebecca L Robker, Robert J Norman, Wayne D Tilley and Theresa E Hickey-
dc.publisherBiomed Central-
dc.rights© 2015 Tuck et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http:// applies to the data made available in this article, unless otherwise stated.-
dc.subjectHuman; ovarian follicles; c-kit; KITL; immunohistochemistry; granulosa; theca; PCOS-
dc.titleExpression and localisation of c-kit and KITL in the adult human ovary-
dc.typeJournal article-
dc.identifier.orcidRobker, R. [0000-0002-1538-4604]-
dc.identifier.orcidNorman, R. [0000-0002-3118-3896]-
dc.identifier.orcidTilley, W. [0000-0003-1893-2626]-
dc.identifier.orcidHickey, T. [0000-0002-2752-730X]-
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