Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/102428
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Type: Journal article
Title: SLAH1, a homologue of the slow type anion channel SLAC1, modulates shoot Cl⁻ accumulation and salt tolerance in Arabidopsis thaliana
Other Titles: SLAH1, a homologue of the slow type anion channel SLAC1, modulates shoot Cl(-) accumulation and salt tolerance in Arabidopsis thaliana
Author: Qiu, J.
Henderson, S.
Tester, M.
Roy, S.
Gilliham, M.
Citation: Journal of Experimental Botany, 2017; 68(12):4495-4505
Publisher: Oxford University Press
Issue Date: 2017
ISSN: 0022-0957
1460-2431
Statement of
Responsibility: 
Jiaen Qiu, Sam W Henderson, Mark Tester, Stuart J Roy and Mathew Gilliham
Abstract: Salinity tolerance is correlated with shoot chloride (Cl⁻) exclusion in multiple crops, but the molecular mechanisms of long-distance Cl⁻ transport are poorly defined. Here, we characterize the in planta role of AtSLAH1 (a homologue of the slow type anion channel-associated 1 (SLAC1)). This protein, localized to the plasma membrane of root stelar cells, has its expression reduced by salt or ABA, which are key predictions for a protein involved with loading Cl– into the root xylem. Artificial microRNA knockdown mutants of AtSLAH1 had significantly reduced shoot Cl− accumulation when grown under low Cl⁻, whereas shoot Cl– increased and the shoot nitrate/chloride ratio decreased following AtSLAH1 constitutive or stelar-specific overexpression when grown in high Cl–. In both sets of overexpression lines a significant reduction in shoot biomass over the null segregants was observed under high Cl⁻ supply, but not low Cl⁻ supply. Further in planta data showed AtSLAH3 overexpression increased the shoot nitrate/chloride ratio, consistent with AtSLAH3 favouring nitrate transport. Heterologous expression of AtSLAH1 in Xenopus laevis oocytes led to no detectible transport, suggesting the need for post-translational modifications for AtSLAH1 to be active. Our in planta data are consistent with AtSLAH1 having a role in controlling root-to-shoot Cl⁻ transport.
Keywords: ABA, Arabidopsis; AtSLAH1, AtSLAH3; chloride; Cl− xylem loading; long-distance transport; nutrition; salinity; slowtype anion channel-associated homologue 1; slow-type anion channel-associated homologue 3
Description: Formally published in vol. 67, no. 15, 2016
Rights: © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License
RMID: 0030050105
DOI: 10.1093/jxb/erw237
Grant ID: http://purl.org/au-research/grants/arc/CE14010008
Published version: http://jxb.oxfordjournals.org/content/67/15/4495
Appears in Collections:Agriculture, Food and Wine publications

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