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Type: Journal article
Title: Quantifying the sensitivity of soil microbial communities to silver sulfide nanoparticles using metagenome sequencing
Author: Doolette, C.
Gupta, V.
Lu, Y.
Payne, J.
Batstone, D.
Kirby, J.
Navarro, D.
McLaughlin, M.
Citation: PLoS One, 2016; 11(8):e0161979-1-e0161979-20
Publisher: Public Library Science
Issue Date: 2016
ISSN: 1932-6203
Editor: Mukherjee, A.
Statement of
Casey L. Doolette, Vadakattu V. S. R. Gupta, Yang Lu, Justin L. Payne, Damien J. Batstone, Jason K. Kirby, Divina A. Navarro, Mike J. McLaughlin
Abstract: Soils are a sink for sulfidised-silver nanoparticles (Ag₂S-NPs), yet there are limited ecotoxicity data for their effects on microbial communities. Conventional toxicity tests typically target a single test species or function, which does not reflect the broader community response. Using a combination of quantitative PCR, 16S rRNA amplicon sequencing and species sensitivity distribution (SSD) methods, we have developed a new approach to calculate silver-based NP toxicity thresholds (HCx, hazardous concentrations) that are protective of specific members (operational taxonomic units, OTUs) of the soil microbial community. At the HC20 (80% of species protected), soil OTUs were significantly less sensitive to Ag₂S-NPs compared to AgNPs and Ag+ (5.9, 1.4 and 1.4 mg Ag kg-1, respectively). However at more conservative HC values, there were no significant differences. These trends in OTU responses matched with those seen in a specific microbial function (rate of nitrification) and amoA-bacteria gene abundance. This study provides a novel molecularbased framework for quantifying the effect of a toxicant on whole soil microbial communities while still determining sensitive genera/species. Methods and results described here provide a benchmark for microbial community ecotoxicological studies and we recommend that future revisions of Soil Quality Guidelines for AgNPs and other such toxicants consider this approach.
Keywords: Bacteria
Silver Compounds
DNA, Bacterial
RNA, Ribosomal, 16S
Sequence Analysis, DNA
Soil Microbiology
Metal Nanoparticles
Rights: Copyright: © 2016 Doolette et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI: 10.1371/journal.pone.0161979
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