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|Title:||Methylation of CpG sites in exon 2 of the Bcl-2 gene occurs in colorectal carcinoma|
|Citation:||Anticancer Research, 2001; 21(4A):2809-2814|
|Publisher:||Int Inst Anticancer Research|
|Babidge WJ; Butler LM; Burton MA; Cowled PA|
|Abstract:||<h4>Background</h4>Aberrant bcl-2 expression frequently occurs in colorectal carcinoma. The current study investigated if CpG sites in bcl-2 were methylated in colorectal carcinoma and if methylation correlated with loss of expression of bcl-2 mRNA.<h4>Methods</h4>Methylation was assessed in 23 matched normal mucosae and colonic carcinomas by Southern blotting with methylation-sensitive enzymes. Expression of bcl-2 mRNA was assessed by Northern blotting.<h4>Results</h4>A SacII site in exon 2 of the bcl-2 gene was methylated in 5 carcinomas, plus an adjacent HpaII sites in 1 tumour. SacII site in the bcl-2 promoter were not methylated. Elevated levels of bcl-2 mRNA were detected in 3 carcinomas, 5 showed decreased expression and 4 were unchanged.<h4>Conclusions</h4>De novo methylation of CpG sites in exon 2 of the bcl-2 gene occurs during the development of colorectal carcinoma. However, there was no relationship between expression of bc1-2 mRNA and methylation of specific CpG sites.|
|Keywords:||Humans; Colorectal Neoplasms; Deoxyribonucleases, Type II Site-Specific; Proto-Oncogene Proteins c-bcl-2; RNA, Messenger; Neoplasm Staging; Blotting, Northern; DNA Methylation; Gene Expression Regulation, Neoplastic; CpG Islands; Genes, bcl-2; Exons; Promoter Regions, Genetic; DNA-Cytosine Methylases|
|Description:||© 2001 International Institute of Anticancer Research|
|Appears in Collections:||Surgery publications|
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