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Type: Journal article
Title: The barley (Hordeum vulgare) cellulose synthase-like D2 gene (HvCslD2) mediates penetration resistance to host-adapted and nonhost isolates of the powdery mildew fungus
Author: Douchkov, D.
Lueck, S.
Hensel, G.
Kumlehn, J.
Rajaraman, J.
Johrde, A.
Doblin, M.
Beahan, C.
Kopischke, M.
Fuchs, R.
Lipka, V.
Niks, R.
Bulone, V.
Chowdhury, J.
Little, A.
Burton, R.
Bacic, A.
Fincher, G.
Schweizer, P.
Citation: New Phytologist, 2016; 212(2):421-433
Publisher: Wiley
Issue Date: 2016
ISSN: 0028-646X
Statement of
Dimitar Douchkov, Stefanie Lueck, Goetz Hensel, Jochen Kumlehn, Jeyaraman Rajaraman, Annika Johrde, Monika S. Doblin, Cherie T. Beahan, Michaela Kopischke, Ren, e Fuchs, Volker Lipka, Rients E. Niks, Vincent Bulone, Jamil Chowdhury, Alan Little, Rachel A. Burton, Antony Bacic, Geoffrey B. Fincher and Patrick Schweizer
Abstract: Cell walls and cellular turgor pressure shape and suspend the bodies of all vascular plants. In response to attack by fungal and oomycete pathogens, which usually breach their host's cell walls by mechanical force or by secreting lytic enzymes, plants often form local cell wall appositions (papillae) as an important first line of defence. The involvement of cell wall biosynthetic enzymes in the formation of these papillae is still poorly understood, especially in cereal crops. To investigate the role in plant defence of a candidate gene from barley (Hordeum vulgare) encoding cellulose synthase-like D2 (HvCslD2), we generated transgenic barley plants in which HvCslD2 was silenced through RNA interference (RNAi). The transgenic plants showed no growth defects but their papillae were more successfully penetrated by host-adapted, virulent as well as avirulent nonhost isolates of the powdery mildew fungus Blumeria graminis. Papilla penetration was associated with lower contents of cellulose in epidermal cell walls and increased digestion by fungal cell wall degrading enzymes. The results suggest that HvCslD2-mediated cell wall changes in the epidermal layer represent an important defence reaction both for nonhost and for quantitative host resistance against nonadapted wheat and host-adapted barley powdery mildew pathogens, respectively.
Keywords: Cell Wall; Ascomycota; Plants, Genetically Modified; Arabidopsis; Hordeum; Plant Epidermis; Glucosyltransferases; Polysaccharides; Plant Proteins; Sequence Analysis, DNA; Plant Diseases; Gene Expression Regulation, Plant; Gene Silencing; Genes, Plant; Host-Pathogen Interactions
Rights: © 2016 The Authors New Phytologist © 2016 New Phytologist Trust
RMID: 0030050101
DOI: 10.1111/nph.14065
Appears in Collections:Agriculture, Food and Wine publications

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