Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/102953
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Type: Journal article
Title: Membrane-mediated protein–protein interactions of cholesterol side-chain cleavage cytochrome P450 with its associated electron transport proteins
Author: Kubeil, C.
Yeung, J.
Tuckey, R.
Rodgers, R.
Martin, L.
Citation: ChemPlusChem, 2016; 81(9):995-1002
Publisher: Wiley-VCH Verlag
Issue Date: 2016
ISSN: 2192-6506
2192-6506
Statement of
Responsibility: 
Clemens Kubeil, Joyee Chun In Yeung, Robert C. Tuckey, Raymond J. Rodgers and Lisandra L. Martin
Abstract: Cytochrome P450scc (P450scc or CYP11A1) catalyses the first enzymatic step of steroid biosynthesis, the cleavage of the side chain of cholesterol to produce pregnenolone in the mitochondrion. The activity of P450scc is dependent upon electron delivery from NADPH-dependent adrenodoxin reductase (AdR), via adrenodoxin (Adx), to the P450scc. However, despite the structural and kinetic data that supports the mechanism by which Adx shuttles electrons one at a time between AdR and the P450scc, there are limited data available on the influence of the lipid membrane on these essential interactions. In this paper, the protein–membrane interactions between P450scc and its redox partners were examined on 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) membranes containing cholesterol (20 %), using a quartz crystal microbalance with dissipation monitoring. P450scc showed strong binding to these membranes, whereas AdR and Adx both showed weaker association. If pre-mixed, all three proteins bound independently to the membrane layer in a distinctive two-stage process, as observed by frequency changes upon binding. Concomitant changes in the dissipation revealed specific protein–protein interaction occurs upon reaching a critical concentration of proteins in the membrane layer. These changes were specific for the binding of the three pre-mixed proteins and were not observed for a binary mixture of P450 and Adx, or sequential binding of the three proteins. A simple model was developed for the binding of all three proteins in a 1:1:1 mixture to the membrane and reproduces the experimental data describing the interaction of P450scc with the other proteins (AdR and Adx) after initial binding of the individual proteins. Thus, we conclude that the lipid membrane assists in the assembly of electron transport proteins and the activity of P450scc by providing a surface for the localised concentration of proteins, enabling them to act together as a metabolon.
Keywords: cytochromes P450
lipids
membranes
protein-protein interactions
quartz crystal microbalance
Rights: © 2016 Wiley-VCH Verlag GmbH &Co. KGaA, Weinheim
DOI: 10.1002/cplu.201600272
Grant ID: ARC
NHMRC
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Obstetrics and Gynaecology publications

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