Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/103066
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Type: Journal article
Title: Development of an improved Streptococcus uberis experimental mastitis challenge model using different doses and strains in lactating dairy cows
Author: Khazandi, M.
Eats, P.
Trott, D.
Ebrahimie, E.
Perry, J.
Hickey, E.
Page, S.
Garg, S.
Petrovski, K.
Citation: Journal of Dairy Research, 2015; 82(4):470-477
Publisher: Cambridge University Press
Issue Date: 2015
ISSN: 0022-0299
1469-7629
Statement of
Responsibility: 
Manouchehr Khazandi, Patricia Eats, Darren Trott, Esmaeil Ebrahimie, Jeanette Perry, Elizabeth Hickey, Stephen Page, Sanjay Garg, and Kiro R Petrovski
Abstract: Developing a reliable mastitis challenge infection model is required to test new intramammary antimicrobial preparations, other novel bovine mastitis treatments, and study mastitis pathogenesis. Three treatment groups of Holstein Friesian cows in active lactation were administered two doses (10⁴ and 10⁶ cfu/quarter) on a single occasion with one of the three Streptococcus uberis strains (BFR6019, MFF1283 and SA002) suspended in 5 ml of sterile PBS, administered via intramammary inoculation immediately after milking. All quarters that were challenged with S. uberis strains MLF1283 and BFR6019 showed clinical signs of mastitis on day 1 and 2 after the challenge. Strain SA002 had a lower rate of inducing clinical mastitis which was detected later than day 3 after the challenge. We successfully developed a rapid and reliable model for inducing experimental S. uberis mastitis with 100% success rate in cows in active lactation. On the basis of the correlation results between strains, RAPD fingerprinting results, clinical findings, and a 100% success rate of mastitis induction for low and high doses S. uberis strains MLF1283 and BFR6019, strain virulence seems to be a more important effect than challenge dose in induction of clinical mastitis following experimental challenge.
Keywords: Mastitis; challenge model; dairy cows; Streptococcus uberis
Rights: © Proprietors of Journal of Dairy Research 2015
RMID: 0030032220
DOI: 10.1017/S0022029915000321
Appears in Collections:Animal and Veterinary Sciences publications

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