Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/103719
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Type: Journal article
Title: Increasing the activity and efficiency of stereoselective oxidations by using decoy molecules in combination with rate-enhancing variants of P450Bm3
Author: Munday, S.
Dezvarei, S.
Bell, S.
Citation: ChemCatChem, 2016; 8(17):2789-2796
Publisher: Wiley
Issue Date: 2016
ISSN: 1867-3899
1867-3899
Statement of
Responsibility: 
Samuel D. Munday, Shaghayegh Dezvarei, Stephen Bell
Abstract: The use of rate-accelerating variants of P450Bm3 coupled with decoy molecules is described, resulting in improved catalytic activity for hydroxylation and epoxidation reactions. Prochiral substrates were investigated to ascertain the effect of the mutant enzymes and the decoy molecules on the regio- and enantioselectivity of the oxidations. For the alkyl and alkene substituted benzene substrates tested, large improvements in the product formation activity over the wild-type enzyme were obtained. The product formation rates for the substrates tested ranged from 660 to 2210 nmol (nmol P450)−1 min−1 for variants containing the R47L and Y51F mutations. Although the regioselectivity was not significantly altered in most of the turnovers, some adjustment in the enantioselectivity was observed with smaller substrates. The addition of decoy molecules often resulted in improved enantioselectivity and counteracted reductions arising from the rate-accelerating mutants.
Keywords: Asymmetric catalysis; C−H bond oxidation; cytochrome P450 monooxygenases; enzyme catalysis; epoxidation
Rights: © 2016 Wiley-VCH Verlag GmbH &Co. KGaA, Weinheim
RMID: 0030052402
DOI: 10.1002/cctc.201600551
Grant ID: http://purl.org/au-research/grants/arc/FT140100355
Appears in Collections:Chemistry and Physics publications

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