Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/10399
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Thiol methyltransferase activity in inflammatory bowel disease |
Author: | Roediger, W. Babidge, W. |
Citation: | Gut, 2000; 47(2):206-210 |
Publisher: | British Med Journal Publ Group |
Issue Date: | 2000 |
ISSN: | 0017-5749 1468-3288 |
Abstract: | <h4>Background</h4>Luminal anionic sulphide may contribute to epithelial damage in ulcerative colitis. Thiol methyltransferase (TMT) governs sulphide detoxification by the colonic mucosa and circulating erythrocytes.<h4>Aims</h4>To measure levels of TMT activity in erythrocytes of surgically treated cases of colitis or in rectal biopsies of defined groups of colitis.<h4>Patients</h4>Venepuncture blood was obtained from 37 blood donors and 27 subjects who had previously undergone a proctocolectomy for colitis: 18 for ulcerative colitis and nine for Crohn's colitis. Rectal biopsies from 122 cases were obtained: 47 without mucosal disease, 33 post-colon resection for cancer, 14 with moderate to severe ulcerative colitis, 15 with quiescent ulcerative colitis, seven with acute Crohn's colitis, and six with radiation proctitis.<h4>Methods</h4>TMT activity was measured by high performance liquid chromatography with radioactive detection to measure (14)C methylmercaptoethanol formation, the reaction product of cell extracts incubated with mercaptoethanol and (14)C S-adenosylmethionine.<h4>Results</h4>Erythrocyte TMT activity of surgically treated cases of colitis was significantly elevated (p<0. 001) compared with control cases. TMT activity of rectal biopsies was significantly decreased (p<0.02) in acute but not quiescent ulcerative colitis, Crohn's colitis, or radiation colitis.<h4>Conclusions</h4>Erythrocyte TMT activity was persistently elevated after proctocolectomy for Crohn's disease and ulcerative colitis. No primary defect of TMT activity was found in any case of unoperated colitis but mucosal activity was diminished with disease progression of ulcerative colitis. Studies of genetic control of TMT activity of erythrocytes in inflammatory bowel disease appear worthwhile. |
Keywords: | Rectum Erythrocytes Humans Colitis, Ulcerative Crohn Disease Methyltransferases Biopsy Chromatography, High Pressure Liquid Analysis of Variance Case-Control Studies Adult Aged Aged, 80 and over Middle Aged Female Male |
DOI: | 10.1136/gut.47.2.206 |
Published version: | http://dx.doi.org/10.1136/gut.47.2.206 |
Appears in Collections: | Aurora harvest 2 Surgery publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.