Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/10399
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Type: Journal article
Title: Thiol methyltransferase activity in inflammatory bowel disease
Author: Roediger, W.
Babidge, W.
Citation: Gut, 2000; 47(2):206-210
Publisher: British Med Journal Publ Group
Issue Date: 2000
ISSN: 0017-5749
1468-3288
Abstract: <h4>Background</h4>Luminal anionic sulphide may contribute to epithelial damage in ulcerative colitis. Thiol methyltransferase (TMT) governs sulphide detoxification by the colonic mucosa and circulating erythrocytes.<h4>Aims</h4>To measure levels of TMT activity in erythrocytes of surgically treated cases of colitis or in rectal biopsies of defined groups of colitis.<h4>Patients</h4>Venepuncture blood was obtained from 37 blood donors and 27 subjects who had previously undergone a proctocolectomy for colitis: 18 for ulcerative colitis and nine for Crohn's colitis. Rectal biopsies from 122 cases were obtained: 47 without mucosal disease, 33 post-colon resection for cancer, 14 with moderate to severe ulcerative colitis, 15 with quiescent ulcerative colitis, seven with acute Crohn's colitis, and six with radiation proctitis.<h4>Methods</h4>TMT activity was measured by high performance liquid chromatography with radioactive detection to measure (14)C methylmercaptoethanol formation, the reaction product of cell extracts incubated with mercaptoethanol and (14)C S-adenosylmethionine.<h4>Results</h4>Erythrocyte TMT activity of surgically treated cases of colitis was significantly elevated (p<0. 001) compared with control cases. TMT activity of rectal biopsies was significantly decreased (p<0.02) in acute but not quiescent ulcerative colitis, Crohn's colitis, or radiation colitis.<h4>Conclusions</h4>Erythrocyte TMT activity was persistently elevated after proctocolectomy for Crohn's disease and ulcerative colitis. No primary defect of TMT activity was found in any case of unoperated colitis but mucosal activity was diminished with disease progression of ulcerative colitis. Studies of genetic control of TMT activity of erythrocytes in inflammatory bowel disease appear worthwhile.
Keywords: Rectum
Erythrocytes
Humans
Colitis, Ulcerative
Crohn Disease
Methyltransferases
Biopsy
Chromatography, High Pressure Liquid
Analysis of Variance
Case-Control Studies
Adult
Aged
Aged, 80 and over
Middle Aged
Female
Male
DOI: 10.1136/gut.47.2.206
Appears in Collections:Aurora harvest 2
Surgery publications

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