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Type: Journal article
Title: Early CCR6 expression on B cells modulates germinal centre kinetics and efficient antibody responses
Author: Reimer, D.
Lee, A.
Bannan, J.
Fromm, P.
Kara, E.
Comerford, I.
McColl, S.
Wiede, F.
Mielenz, D.
Körner, H.
Citation: Immunology and Cell Biology, 2017; 95(1):33-41
Publisher: Nature Publishing
Issue Date: 2017
ISSN: 0818-9641
Statement of
Dorothea Reimer, Adrian YS Lee, Jennifer Bannan, Phillip Fromm, Ervin E Kara, Iain Comerford, Shaun McColl, Florian Wiede, Dirk Mielenz and Heinrich Körner
Abstract: The CC-chemokine receptor 6 (CCR6) can be detected on naïve and activated B cells. Counterintuitively, its absence accelerates the appearance of germinal centres (GC) and increases the production of low-affinity antibodies. The detailed mechanism of CCR6 function during the humoral response has remained elusive but previously we identified a distinct CCR6(high) B cell population in vivo early after antigenic challenge. In this study, we defined this population specifically as early, activated pre-GC B cells. In accordance, we show that CCR6 is up-regulated rapidly within hours on protein or mRNA level after activation in vitro. Additionally, only activated B cells migrated specifically towards CCL20, the specific ligand for CCR6. Lack of CCR6 increased the dark zone/light zone ratio of GC and led to decreased antigen-specific IgG1 and IgG2a antibody generation in a B cell intrinsic manner in mixed bone marrow chimeras. In contrast, antigen-specific IgM responses were normal. Hence, CCR6 negatively regulates entry of activated, antigen-specific pre-GC B cells into the GC reaction.
Keywords: Germinal Center
Mice, Inbred C57BL
RNA, Messenger
Flow Cytometry
Lymphocyte Activation
Cell Movement
Antibody Formation
Receptors, CCR6
Chemokine CCL20
Rights: © 2017 Australasian Society for Immunology Inc. All rights reserved
DOI: 10.1038/icb.2016.68
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Molecular and Biomedical Science publications

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