Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/104372
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Type: Journal article
Title: F-actin distribution and function during sexual development in Eimeria maxima
Author: Frölich, S.
Wallach, M.
Citation: Parasitology, 2015; 142(7):855-864
Publisher: Cambridge University Press
Issue Date: 2015
ISSN: 0031-1820
1469-8161
Statement of
Responsibility: 
Sonja Frölich nd Michael Wallach
Abstract: To determine the involvement of the actin cytoskeleton in macrogametocyte growth and oocyst wall formation, freshly purified macrogametocytes and oocysts were stained with Oregon Green 514 conjugated phalloidin to visualize F-actin microfilaments, while Evans blue staining was used to detect type 1 wall forming bodies (WFB1s) and the outer oocyst wall. The double-labelled parasites were then analysed at various stages of sexual development using three-dimensional confocal microscopy. The results showed F-actin filaments were distributed throughout the entire cytoplasm of mature Eimeria maxima macrogametocytes forming a web-like meshwork of actin filaments linking the type 1 WFBs together into structures resembling 'beads on a string'. At the early stages of oocyst wall formation, F-actin localization changed in alignment with the egg-shaped morphology of the forming oocysts with F-actin microfilaments making direct contact with the WFB1s. In tissue oocysts, the labelled actin cytoskeleton was situated underneath the forming outer layer of the oocyst wall. Treatment of macrogametocytes in vitro with the actin depolymerizing agents, Cytochalasin D and Latrunculin, led to a reduction in the numbers of mature WFB1s in the cytoplasm of the developing macrogametocytes, indicating that the actin plays an important role in WFB1 transport and oocyst wall formation in E. maxima.
Keywords: Actin; cytoskeletal inhibitors; WFB1 vesicle transport; Eimeria oocyst; 3D confocal microscopy
Rights: © Cambridge University Press 2015
RMID: 0030045915
DOI: 10.1017/S0031182015000207
Appears in Collections:Animal and Veterinary Sciences publications

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