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Type: Journal article
Title: Differences in glycosyltransferase family 61 accompany variation in seed coat mucilage composition in Plantago spp.
Author: Phan, J.
Tucker, M.
Khor, S.
Shirley, N.
Lahnstein, J.
Beahan, C.
Bacic, A.
Burton, R.
Citation: Journal of Experimental Botany, 2016; 67(22):6481-6495
Publisher: Oxford University Press
Issue Date: 2016
ISSN: 0022-0957
Statement of
Jana L. Phan, Matthew R. Tucker, Shi Fang Khor, Neil Shirley, Jelle Lahnstein, Cherie Beahan, Antony Bacic and Rachel A. Burton
Abstract: Xylans are the most abundant non-cellulosic polysaccharide found in plant cell walls. A diverse range of xylan structures influence tissue function during growth and development. Despite the abundance of xylans in nature, details of the genes and biochemical pathways controlling their biosynthesis are lacking. In this study we have utilized natural variation within the Plantago genus to examine variation in heteroxylan composition and structure in seed coat mucilage. Compositional assays were combined with analysis of the glycosyltransferase family 61 (GT61) family during seed coat development, with the aim of identifying GT61 sequences participating in xylan backbone substitution. The results reveal natural variation in heteroxylan content and structure, particularly in P. ovata and P. cunninghamii, species which show a similar amount of heteroxylan but different backbone substitution profiles. Analysis of the GT61 family identified specific sequences co-expressed with IRREGULAR XYLEM 10 genes, which encode putative xylan synthases, revealing a close temporal association between xylan synthesis and substitution. Moreover, in P. ovata, several abundant GT61 sequences appear to lack orthologues in P. cunninghamii. Our results indicate that natural variation in Plantago species can be exploited to reveal novel details of seed coat development and polysaccharide biosynthetic pathways.
Keywords: Glycosyltransferase family 61; heteroxylan; Plantago; plant cell walls; polysaccharide biosynthesis; seed mucilage
Rights: © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
DOI: 10.1093/jxb/erw424
Grant ID: ARC
Appears in Collections:Agriculture, Food and Wine publications
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