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Type: Theses
Title: Characterisation of glutamine synthetase to map new regulatory loci modulating nitrogen use efficiency in hexaploid wheat
Author: Djietror, Jonathan Castel
Issue Date: 2016
School/Discipline: School of Agriculture, Food and Wine
Abstract: The main aim of this research project is to identify and characterise genetic variation within a diverse collection of wheat germplasm for key enzymes linked with N metabolism in wheat and related cereal species. Moreover, the study will attempt to address the following research objectives: • Identity and confirm the genetic loci of GS homologues in hexaploid wheat genome. • Characterise sequence diversity among different accessions of wheat through phylogenetic analysis. • Develop molecular markers within the GS conserved domain sequences. • Assess GS expression by quantifying the transcript abundance under high and low N treatment of wheat plants. • Evaluate effects of genetic variation on GS activity under high and low N. The germplasm for this project is sourced from a genetically diverse pool of wheat, adapted for the growth in the major cultivation zones around the world, and represent a unique resource for use in this study.
Advisor: Chalmers, Kenneth James
Mather, Diane Elizabeth
Heuer, Sigrid
Plett, Darren Craig
Croxford, Adam
Taylor, Julian
Dissertation Note: Thesis (Ph.D.) (Research by Publication) -- University of Adelaide,School of Agriculture, Food and Wine, 2016.
Keywords: glutamine synthetase
nitrogen use efficiency
genetic variation
hexaploid wheat
Research by Publication
Provenance: This electronic version is made publicly available by the University of Adelaide in accordance with its open access policy for student theses. Copyright in this thesis remains with the author. This thesis may incorporate third party material which has been used by the author pursuant to Fair Dealing exceptions. If you are the owner of any included third party copyright material you wish to be removed from this electronic version, please complete the take down form located at:
DOI: 10.4225/55/591d08e2dee13
Appears in Collections:Research Theses

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