Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/105295
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Type: Journal article
Title: Matrilin-3 chondrodysplasia mutations cause attenuated chondrogenesis, premature hypertrophy and aberrant response to TGF-β in chondroprogenitor cells
Other Titles: Matrilin-3 chondrodysplasia mutations cause attenuated chondrogenesis, premature hypertrophy and aberrant response to TGF-beta in chondroprogenitor cells
Author: Jayasuriya, C.
Zhou, F.
Pei, M.
Wang, Z.
Lemme, N.
Haines, P.
Chen, Q.
Citation: International Journal of Molecular Sciences, 2014; 15(8):14555-14573
Publisher: MDPI AG
Issue Date: 2014
ISSN: 1422-0067
1422-0067
Statement of
Responsibility: 
Chathuraka T. Jayasuriya, Fiona H. Zhou, Ming Pei, Zhengke Wang, Nicholas J. Lemme, Paul Haines and Qian Chen
Abstract: Studies have shown that mutations in the matrilin-3 gene (MATN3) are associated with multiple epiphyseal dysplasia (MED) and spondyloepimetaphyseal dysplasia (SEMD). We tested whether MATN3 mutations affect the differentiation of chondroprogenitor and/or mesenchymal stem cells, which are precursors to chondrocytes. ATDC5 chondroprogenitors stably expressing wild-type (WT) MATN3 underwent spontaneous chondrogenesis. Expression of chondrogenic markers collagen II and aggrecan was inhibited in chondroprogenitors carrying the MED or SEMD MATN3 mutations. Hypertrophic marker collagen X remained attenuated in WT MATN3 chondroprogenitors, whereas its expression was elevated in chondroprogenitors expressing the MED or SEMD mutant MATN3 gene suggesting that these mutations inhibit chondrogenesis but promote hypertrophy. TGF-β treatment failed to rescue chondrogenesis markers but dramatically increased collagen X mRNA expression in mutant MATN3 expressing chondroprogenitors. Synovium derived mesenchymal stem cells harboring the SEMD mutation exhibited lower glycosaminoglycan content than those of WT MATN3 in response to TGF-β. Our results suggest that the properties of progenitor cells harboring MATN3 chondrodysplasia mutations were altered, as evidenced by attenuated chondrogenesis and premature hypertrophy. TGF-β treatment failed to completely rescue chondrogenesis but instead induced hypertrophy in mutant MATN3 chondroprogenitors. Our data suggest that chondroprogenitor cells should be considered as a potential target of chondrodysplasia therapy.
Keywords: Matrilin-3; multiple epiphyseal dysplasia; spondyloepimetaphyseal dysplasia; chondroprogenitor; chondrogenesis; TGF-β
Description: Published: 21 August 2014
Rights: © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
DOI: 10.3390/ijms150814555
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