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Type: Journal article
Title: Inhibitory responses mediated by vagal nerve stimulation are diminished in stomachs of mice with reduced intramuscular interstitial cells of Cajal
Author: Beckett, E.
Sanders, K.
Ward, S.
Citation: Scientific Reports, 2017; 7(1):1-11
Publisher: Nature Publishing Group
Issue Date: 2017
ISSN: 2045-2322
Statement of
Elizabeth A. H. Beckett, Kenton M. Sanders and Sean M. Ward
Abstract: Intramuscular interstitial cells of Cajal (ICC-IM) are closely associated with enteric motor nerve terminals and electrically coupled to smooth muscle cells within the gastric musculature. Previous studies investigating the role of ICC-IM in motor neurotransmission have used indiscriminate electric field stimulation of neural elements within the gastric wall. To determine the role of ICC-IM in transduction of vagally-mediated motor input to gastric muscles electrical and mechanical responses to selective electrical vagal stimulation (EVS) were recorded from gastric fundus and antral regions of wild type and W/WV mice, which lack most ICC-IM. EVS evoked inhibitory junction potentials (IJPs) in wild type muscles that were attenuated or abolished by L-NNA. IJPs were rarely evoked in W/WV muscles by EVS, and not affected by L-NNA. EVS evoked relaxation of wild type stomachs, but the predominant response of W/WV stomachs was contraction. EVS applied after pre-contraction with bethanechol caused relaxation of wild type gastric tissues and these were inhibited by the nitric oxide synthase inhibitor L-NNA. Relaxation responses were of smaller amplitude in W/WV muscles and L-NNA did not attenuate relaxation responses in W/WV fundus muscles. These data suggest an important role for ICC-IM in vagally-mediated nitrergic relaxation in the proximal and distal stomach.
Keywords: Muscle, Smooth; Stomach; Neuromuscular Junction; Animals; Mice, Inbred C57BL; Hexamethonium; Receptors, Nicotinic; Nicotinic Antagonists; Electric Stimulation; Muscle Relaxation; Nitric Oxide Synthase; Vagus Nerve Stimulation; Interstitial Cells of Cajal
Rights: © The Author(s) 2017. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit
RMID: 0030068215
DOI: 10.1038/srep44759
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Appears in Collections:Medicine publications

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