Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/105834
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Antimicrobial resistance in coagulase-positive staphylococci isolated from companion animals in Australia: a one year study
Author: Saputra, S.
Jordan, D.
Worthing, K.
Norris, J.
Wong, H.
Abraham, R.
Trott, D.
Abraham, S.
Citation: PLoS ONE, 2017; 12(4):e0176379-1-e0176379-17
Publisher: Public Library of Science (PLoS)
Issue Date: 2017
ISSN: 1932-6203
1932-6203
Statement of
Responsibility: 
Sugiyono Saputra, David Jordan, Kate A. Worthing, Jacqueline M. Norris, Hui S. Wong, Rebecca Abraham, Darren J. Trott, Sam Abraham
Abstract: Methicillin-resistant coagulase-positive staphylococci (CoPS) have become increasingly recognised as opportunistic pathogens that limit therapeutic options in companion animals. The frequency of methicillin resistance amongst clinical isolates on an Australia-wide level is unknown. This study determined antimicrobial susceptibility patterns for CoPS isolated from clinical infections in companion animals (dogs, cats and horses) as part of the first nation-wide survey on antimicrobial resistance in animal pathogens in Australia for a one-year period (January 2013 to January 2014). Clinical Staphylococcus spp. isolates (n = 888) obtained from 22 veterinary diagnostic laboratories were identified by MALDI-TOF mass spectrometry and subjected to antimicrobial susceptibility testing for 16 antimicrobials, representing 12 antimicrobial classes. Potential risk factors associated with methicillin resistance in Staphylococcus pseudintermedius isolates from dogs were analysed based on demographic factors and clinical history, including gender, age, previous antimicrobial treatment, chronic and/or recurrent diseases and site of infections. The most commonly identified CoPS were S. pseudintermedius (70.8%; dogs n = 616, cats n = 13) and S. aureus (13.2%, horses n = 53, dogs n = 47 and cats n = 17). Overall, the frequency of methicillin resistance among S. pseudintermedius (MRSP) and S. aureus (MRSA) was 11.8% and 12.8%, respectively. MRSP isolates were strongly associated with resistance to fluoroquinolones (OR 287; 95%CI 91.2-1144.8) and clindamycin (OR 105.2, 95%CI 48.5-231.9). MRSA isolates from dogs and cats were also more likely to be resistant to fluoroquinolones (OR 5.4, 95%CI 0.6-252.1), whereas MRSA from horses were more likely to be resistant to rifampicin. In multivariate analysis, MRSP-positive status was significantly associated with particular infection sites, including surgical (OR 8.8; 95%CI 3.74-20.7), and skin and soft tissue (OR 3.9; 95%CI 1.97-7.51). S. pseudintermedius isolated from dogs with surgical site infections were three times more likely to be methicillin-resistant if cases had received prior antimicrobial treatment. Whilst the survey results indicate the proportion of CoPS obtained from Australian companion animals that are methicillin-resistant is currently moderate, the identified risk factors suggest that it could rapidly increase without adequate biosecurity and infection control procedures in veterinary practice.
Keywords: Animals; Dogs; Cats; Staphylococcus aureus; Coagulase; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Microbial Sensitivity Tests; Australia
Description: Published: April 21, 2017
Rights: © 2017 Saputra et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
RMID: 0030069146
DOI: 10.1371/journal.pone.0176379
Grant ID: http://purl.org/au-research/grants/arc/LP130100736
Appears in Collections:Animal and Veterinary Sciences publications

Files in This Item:
File Description SizeFormat 
hdl_105834.pdfPublished Version930.75 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.