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|Title:||Revegetation rewilds the soil bacterial microbiome of an old field|
|Citation:||Molecular Ecology, 2017; 26(11):2895-2904|
|Nicholas J. C. Gellie, Jacob G. Mills, Martin F. Breed and Andrew J. Lowe|
|Abstract:||Ecological restoration is a globally important and well-ﬁnanced management interven- tion used to combat biodiversity declines and land degradation. Most restoration aims to increase biodiversity towards a reference state, but there are concerns that intended outcomes are not reached due to unsuccessful interventions and land-use legacy issues. Monitoring biodiversity recovery is essential to measure success; however, most projects remain insufﬁciently monitored. Current ﬁeld-based methods are hard to standardize and are limited in their ability to assess important components of ecosystems, such as bacteria. High-throughput amplicon sequencing of environmental DNA (metabarcoding of eDNA) has been proposed as a cost-effective, scalable and uniform ecological moni- toring solution, but its application in restoration remains largely untested. Here we show that metabarcoding of soil eDNA is effective at demonstrating the return of the native bacterial community in an old ﬁeld following native plant revegetation. Bacterial composition shifted signiﬁcantly after 8 years of revegetation, where younger sites were more similar to cleared sites and older sites were more similar to remnant stands. Revegetation of the native plant community strongly impacted on the belowground bac- terial community, despite the revegetated sites having a long and dramatically altered land-use history (i.e. >100 years grazing). We demonstrate that metabarcoding of eDNA provides an effective way of monitoring changes in bacterial communities that would otherwise go unchecked with conventional monitoring of restoration projects. With fur- ther development, awareness of microbial diversity in restoration has signiﬁcant scope for improving the efﬁcacy of restoration interventions more broadly.|
|Keywords:||Anthropocene; ecosystem restoration; eDNA ; land degradation; microbiome; next-generation sequencing|
|Rights:||© 2017 John Wiley & Sons Ltd|
|Appears in Collections:||Environment Institute publications|
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