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Type: Journal article
Title: Transient dominant host-range selection using Chinese hamster ovary cells to generate marker-free recombinant viral vectors from Vaccinia virus
Author: Liu, L.
Cooper, T.
Eldi, P.
Garcia-Valtanen, P.
Diener, K.
Howley, P.
Hayball, J.
Citation: BioTechniques, 2017; 62(4):183-187
Publisher: BioTechniques
Issue Date: 2017
ISSN: 0736-6205
Statement of
Liang Liu, Tamara Cooper, Preethi Eldi, Pablo Garcia- Valtanen, Kerrilyn R. Diener, Paul M. Howley, and John D. Hayball
Abstract: Recombinant vaccinia viruses (rVACVs) are promising antigen-delivery systems for vaccine development that are also useful as research tools. Two common methods for selection during construction of rVACV clones are (i) co-insertion of drug resistance or reporter protein genes, which requires the use of additional selection drugs or detection methods, and (ii) dominant host-range selection. The latter uses VACV variants rendered replication-incompetent in host cell lines by the deletion of host-range genes. Replicative ability is restored by co-insertion of the host-range genes, providing for dominant selection of the recombinant viruses. Here, we describe a new method for the construction of rVACVs using the cowpox CP77 protein and unmodified VACV as the starting material. Our selection system will expand the range of tools available for positive selection of rVACV during vector construction, and it is substantially more high-fidelity than approaches based on selection for drug resistance.
Keywords: Recombinant viral vectors
Rights: Copyright status unknown
RMID: 0030068130
DOI: 10.2144/000114537
Grant ID:
Appears in Collections:Medicine publications

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