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|Title:||DR5 and caspase-8 are dispensable in ER stress-induced apoptosis|
|Citation:||Cell Death and Differentiation, 2017; 24(5):944-950|
|Publisher:||Nature Publishing Group|
|Jason A Glab, Marcel Doerflinger, Christina Nedeva, Irvin Jose, George W Mbogo, James C Paton, Adrienne W Paton, Andrew J Kueh, Marco J Herold, David CS Huang, David Segal, Gabriella Brumatti, and Hamsa Puthalakath|
|Abstract:||The endoplasmic reticulum (ER) stress response constitutes cellular reactions triggered by a wide variety of stimuli that disturb folding of proteins, often leading to apoptosis. ER stress-induced apoptotic cell death is thought to be an important contributor to many human pathological conditions. The molecular mechanism of this apoptosis process has been highly controversial with both the receptor and the mitochondrial pathways being implicated. Using knockout mouse models and RNAi-mediated gene silencing in cell lines, our group and others had demonstrated the importance of the mitochondrial apoptotic pathway in ER stress-induced cell death, particularly the role of the pro-apoptotic BH3-only BCL-2 family members, BIM and PUMA. However, a recent report suggested a central role for the death receptor, DR5, activated in a ligand-independent manner, and the initiator caspase, caspase-8, in ER stress-induced cell death. This prompted us to re-visit our previous observations and attempt to reproduce the newly published findings. Here we report that the mitochondrial apoptotic pathway, activated by BH3-only proteins, is essential for ER stress-induced cell death and that, in contrast to the previous report, DR5 as well as caspase-8 are not required for this process.|
|Keywords:||Cell Line, Transformed; HCT116 Cells; Endoplasmic Reticulum; Mitochondria; Fibroblasts; Animals; Mice, Knockout; Humans; Mice; Thapsigargin; Brefeldin A; Proto-Oncogene Proteins; Tunicamycin; Signal Transduction; Apoptosis; Gene Expression Regulation; Apoptosis Regulatory Proteins; Caspase 8; Receptors, TNF-Related Apoptosis-Inducing Ligand; Endoplasmic Reticulum Stress; MCF-7 Cells; Bcl-2-Like Protein 11|
|Rights:||© 2017 Macmillan Publishers Limited, part of Springer Nature. All rights reserved|
|Appears in Collections:||Environment Institute publications|
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