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Type: Journal article
Title: Molecular and serological survey of selected viruses in free-ranging wild ruminants in Iran
Author: Hemmatzadeh, F.
Boardman, W.
Alinejad, A.
Hematzade, A.
Moghadam, M.
Citation: PLoS One, 2016; 11(12):1-14
Publisher: Graciela Andrei, Katholieke Universiteit Leuven Rega Institute for Medical Research, BELGIUM
Issue Date: 2016
ISSN: 1932-6203
Statement of
Farhid Hemmatzadeh, Wayne Boardman, Arezo Alinejad, Azar Hematzade, Majid Kharazian Moghadam
Abstract: A molecular and serological survey of selected viruses in free-ranging wild ruminants was conducted in 13 different districts in Iran. Samples were collected from 64 small wild ruminants belonging to four different species including 25 Mouflon (Ovis orientalis), 22 wild goat (Capra aegagrus), nine Indian gazelle (Gazella bennettii) and eight Goitered gazelle (Gazella subgutturosa) during the national survey for wildlife diseases in Iran. Serum samples were evaluated using serologic antibody tests for Peste de petits ruminants virus (PPRV), Pestiviruses [Border Disease virus (BVD) and Bovine Viral Diarrhoea virus (BVDV)], Bluetongue virus (BTV), Bovine herpesvirus type 1 (BHV-1), and Parainfluenza type 3 (PI3). Sera were also ELISA tested for Pestivirus antigen. Tissue samples including spleen, liver, lung, tonsils, mesenteric and mediastinal lymph nodes and white blood cells (WBCs) were tested using polymerase chain reaction (PCR) for PPRV, Foot and Mouth Disease virus (FMDV), Pestivirus, BTV, Ovine herpesvirus type 2 (OvHV-2) and BHV-1. Serologic tests were positive for antibodies against PPRV (17%), Pestiviruses (2%) and BTV (2%). No antibodies were detected for BHV-1 or PI3, and no Pestivirus antigen was detected. PCR results were positive for PPRV (7.8%), FMDV (11%), BTV (3%), OvHV-2 (31%) and BHV-1 (1.5%). None of the samples were positive for Pestiviruses.
Keywords: Animals
Virus Diseases
Antibodies, Viral
Polymerase Chain Reaction
Rights: © 2016 Hemmatzadeh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI: 10.1371/journal.pone.0168756
Appears in Collections:Animal and Veterinary Sciences publications
Aurora harvest 8

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