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Type: Journal article
Title: Accumulation of promutagenic DNA adducts in the mouse distal colon after consumption of heme does not induce colonic neoplasms in the western diet model of spontaneous colorectal cancer
Author: Winter, J.
Young, G.
Hu, Y.
Gratz, S.
Conlon, M.
Le Leu, R.
Citation: Molecular Nutrition and Food Research, 2014; 58(3):550-558
Publisher: Wiley-Blackwell
Issue Date: 2014
ISSN: 1613-4125
Statement of
Jean Winter, Graeme P. Young, Ying Hu, Silvia W. Gratz, Michael A. Conlon and Richard K. Le Leu
Abstract: Red meat is considered a risk factor for colorectal cancer (CRC). Heme is considered to promote colonic hyperproliferation and cell damage. Resistant starch (RS) is a food that ferments in the colon with studies demonstrating protective effects against CRC. By utilizing the western diet model of spontaneous CRC, we determined if feeding heme (as hemin chloride) equivalent to a high red meat diet would increase colonic DNA adducts and CRC and whether RS could abrogate such effects.Four groups of mice: control, heme, RS and heme + RS were fed diets for 1 or 18 months. Colons were analyzed for apoptosis, proliferation, DNA adducts "8-hydroxy-2-deoxyguanosine" and "O(6) -methyl-2-deoxyguanosine" (O(6) MeG), and neoplasms. In the short term, heme increased cell proliferation (p < 0.05). Changes from 1 to 18 months showed increased cell proliferation (p < 0.01) and 8-hydroxy-2-deoxyguanosine adducts (p < 0.05) in all groups, but only heme-fed mice showed reduced apoptosis (p < 0.01) and increased O(6) MeG adducts (p < 0.01). The incidence of colon neoplasms was not different between any interventions.We identified heme to increase proliferation in the short term, inhibit apoptosis over the long term, and increase O(6) MeG adducts in the colon over time although these changes did not affect colonic neoplasms within this mouse model.
Keywords: Heme; 8-hydroxy-2-deoxyguanosine; O6-methyl-2-deoxyguanosine; red meat; resistant starch
Rights: © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
RMID: 0030072212
DOI: 10.1002/mnfr.201300430
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Appears in Collections:Medicine publications

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